FastQCFastQC Report
Tue 22 Aug 2017
HGGK5BGX3_n01_126.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGK5BGX3_n01_126.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18159
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1991.0958753235310315TruSeq Adapter, Index 19 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG1841.0132716559281898TruSeq Adapter, Index 20 (98% over 63bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCAAATCTCGTATGCCGTCTTCTGCTTGAA920.5066358279640949TruSeq Adapter, Index 19 (97% over 39bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG680.37446995979954845No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAG530.29186629219670684TruSeq Adapter, Index 3 (97% over 37bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCAAATCTCGTATGCCGTCTTCTGCTTG420.2312902692879564TruSeq Adapter, Index 19 (97% over 39bp)
ACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCAAATCTCGTATGCCGTCTTCTGCTTGA280.15419351285863758TruSeq Adapter, Index 19 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG240.1321658681645465TruSeq Adapter, Index 15 (98% over 63bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG210.1156451346439782TruSeq Adapter, Index 6 (100% over 63bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGA252.500259E-455.230
AAAAAGG352.1849024E-549.28571369
CTCTTCC557.000992E-637.636361
TCTTCCG655.068141E-737.153852
TTCCGAT601.2714183E-534.54
TCCGATC601.2714183E-534.55
CCGATCT601.2714183E-534.56
CTTCCGA601.2714183E-534.53
GAAAAAA1451.8189894E-1128.55172564
TTGAAAA1352.582965E-1028.1111162
TGAAAAA1352.582965E-1028.1111163
AAAAAAG1253.607056E-927.669
CTTGAAA1455.9480953E-1026.17241561
CATCTCG700.001283980624.64285740
CACGTGG902.0165123E-423.032
TCACGTG952.90793E-421.78947431
GCTTGAA1709.4558345E-820.29411760
ACGTGGC850.00396845820.29411733
GCACACG3700.018.6486512
AAGAGCA3900.018.5769238
Produced by FastQC (version 0.11.2)