FastQCFastQC Report
Tue 22 Aug 2017
HGGK5BGX3_n01_125.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGK5BGX3_n01_125.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17882555
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG8160914.563615210466289TruSeq Adapter, Index 12 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG338740.18942483330821575No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGAA1205200.052.20672660
TGCTTGA1218000.051.5702859
AAAAAAG1226350.050.8608869
CTTGAAA1293550.048.77967561
CTGCTTG1308800.047.99791758
GAAAAAA1339400.047.4308964
TGAAAAA1361500.046.5369663
TTGAAAA1376100.045.97340862
TCGGGGG20250.044.1258244
TCTGCTT1445200.043.532557
CCGTCTT1469500.042.82425751
GCCGTCT1471050.042.77035550
CTTCTGC1485400.042.4472755
CGTCTTC1504400.041.88377452
TTCTGCT1505200.041.8475356
TGCCGTC1531350.041.11818349
GTCTTCT1538500.040.99580453
CGGGGGG22000.040.9292225
TCTTCTG1551250.040.62104854
ATGCCGT1563700.040.28495448
Produced by FastQC (version 0.11.2)