FastQCFastQC Report
Tue 22 Aug 2017
HGGK5BGX3_n01_124.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGK5BGX3_n01_124.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15050283
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG7546015.01386585222351TruSeq Adapter, Index 11 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG287810.19123228446933524No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGAA1111950.052.4705160
TGCTTGA1124650.051.70024559
AAAAAAG1124300.051.27617669
CTGCTTG1173500.049.4775358
CTTGAAA1194800.048.953261
GAAAAAA1246400.047.36572364
TGAAAAA1261600.046.639863
TTGAAAA1267200.046.2868462
TCTGCTT1285900.045.20131357
GCCGTCT1291800.045.09457850
CCGTCTT1296000.044.9401551
TCGGGGG15400.044.356944
CTTCTGC1317050.044.19495855
CGTCTTC1326650.043.92529352
TTCTGCT1336400.043.4981156
TGCCGTC1345450.043.3736449
GTCTTCT1351150.043.12384453
ATGCCGT1370150.042.62712548
TCTTCTG1370550.042.44014754
CGGGGGG17150.042.0435375
Produced by FastQC (version 0.11.2)