FastQCFastQC Report
Tue 22 Aug 2017
HGGK5BGX3_n01_122.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGK5BGX3_n01_122.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15671079
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG6346454.04978495737275TruSeq Adapter, Index 10 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG309960.19779110296106606No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGAA980300.049.8534160
TGCTTGA990550.049.13205359
AAAAAAG1009300.048.1915169
CTGCTTG1043100.046.58739558
CTTGAAA1062250.046.15009761
TCGGGGG16200.045.149164
GAAAAAA1117150.044.37567564
TGAAAAA1143100.043.2811863
TTGAAAA1143250.043.09444462
TCTGCTT1166550.041.82020657
CCGTCTT1179800.041.4072851
GCCGTCT1179800.041.37511450
CGGGGGG18350.041.175315
CTTCTGC1199050.040.70106555
CGTCTTC1203550.040.586852
TTCTGCT1211400.040.23745356
TGCCGTC1228850.039.81651749
GTCTTCT1233900.039.55214353
ATGCCGT1257700.038.93871348
TCTTCTG1253300.038.9341654
Produced by FastQC (version 0.11.2)