FastQCFastQC Report
Tue 22 Aug 2017
HGGK5BGX3_n01_121.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGK5BGX3_n01_121.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18923912
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG4967082.6247638437549274TruSeq Adapter, Index 7 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG218000.11519816832798631No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGAA769000.050.58677760
TGCTTGA767450.050.42386259
CTGCTTG813750.047.51660558
CTTGAAA879200.044.49337861
GCCGTCT890850.043.71505450
CCGTCTT898550.043.35523251
AAAAAAG908600.042.55214769
TGCCGTC934750.041.84318549
TCTGCTT933150.041.58456457
CTTCTGC939250.041.40609755
CGTCTTC943700.041.3649352
TGAAAAA973050.040.64874663
ATGCCGT968850.040.41685548
TTGAAAA981450.040.10399662
GAAAAAA997700.039.90001364
TTCTGCT976100.039.75113356
GTCTTCT992750.039.29347253
TATGCCG1017900.038.47245847
TCTTCTG1022500.038.11299554
GTATGCC1032900.037.94715546
Produced by FastQC (version 0.11.2)