FastQCFastQC Report
Tue 22 Aug 2017
HGGK5BGX3_n01_120.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGK5BGX3_n01_120.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19182912
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG8077954.211013426949985TruSeq Adapter, Index 6 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG291520.15196858537431648No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGAA1171050.052.63289660
TGCTTGA1182850.051.93005859
AAAAAAG1222950.049.99519369
CTTGAAA1254750.049.24540761
CTGCTTG1263700.048.533858
TGAAAAA1305850.047.57412363
TTGAAAA1317600.047.00872462
GAAAAAA1341200.046.49254664
TCTGCTT1435250.042.82659557
TCGGGGG20350.042.7227944
CCGTCTT1447400.042.4919251
CTTCTGC1457650.042.2464655
GCCGTCT1464850.042.0072650
CGTCTTC1474800.041.7561752
CGGGGGG21650.041.1135755
TGCCGTC1511650.040.7893249
TTCTGCT1511700.040.70401456
TCTTCTG1514350.040.62886454
GTCTTCT1537250.040.04168353
ATGCCGT1545500.039.93612748
Produced by FastQC (version 0.11.2)