FastQCFastQC Report
Wed 1 Feb 2017
HGGHHBGX2_n01_9w-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGHHBGX2_n01_9w-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10080680
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG610280.6053956677525723RNA PCR Primer, Index 33 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGGCG85050.059.91061833
CGTATGC85850.059.5940344
TATGCCG86350.059.13056646
GTATGCC89850.056.74928745
CAGGCGA88650.056.5042534
ATGCCGT90450.056.2603747
TCGTATG92050.055.50485643
GCACACG115850.055.29631811
ACGTCTG115400.055.2722515
CACACGT117100.054.61739712
ACCAGGC94000.054.2433332
CTCGTAT92200.052.83242
ACACGTC122350.052.33017713
AGGCGAT94650.052.19237535
GGCGATC91600.051.70849236
CACGTCT124350.051.37729614
GCCGTCT99850.050.79144349
AGCACAC127000.050.46872310
GCGATCT94750.049.88019637
TCTCGTA98400.048.97678441