Basic Statistics
Measure | Value |
---|---|
Filename | HGGHHBGX2_n01_9w-8.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 10080680 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG | 61028 | 0.6053956677525723 | RNA PCR Primer, Index 33 (100% over 43bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CCAGGCG | 8505 | 0.0 | 59.910618 | 33 |
CGTATGC | 8585 | 0.0 | 59.59403 | 44 |
TATGCCG | 8635 | 0.0 | 59.130566 | 46 |
GTATGCC | 8985 | 0.0 | 56.749287 | 45 |
CAGGCGA | 8865 | 0.0 | 56.50425 | 34 |
ATGCCGT | 9045 | 0.0 | 56.26037 | 47 |
TCGTATG | 9205 | 0.0 | 55.504856 | 43 |
GCACACG | 11585 | 0.0 | 55.296318 | 11 |
ACGTCTG | 11540 | 0.0 | 55.27225 | 15 |
CACACGT | 11710 | 0.0 | 54.617397 | 12 |
ACCAGGC | 9400 | 0.0 | 54.24333 | 32 |
CTCGTAT | 9220 | 0.0 | 52.832 | 42 |
ACACGTC | 12235 | 0.0 | 52.330177 | 13 |
AGGCGAT | 9465 | 0.0 | 52.192375 | 35 |
GGCGATC | 9160 | 0.0 | 51.708492 | 36 |
CACGTCT | 12435 | 0.0 | 51.377296 | 14 |
GCCGTCT | 9985 | 0.0 | 50.791443 | 49 |
AGCACAC | 12700 | 0.0 | 50.468723 | 10 |
GCGATCT | 9475 | 0.0 | 49.880196 | 37 |
TCTCGTA | 9840 | 0.0 | 48.976784 | 41 |