FastQCFastQC Report
Wed 1 Feb 2017
HGGHHBGX2_n01_9w-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGHHBGX2_n01_9w-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12760461
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA792840.6213255148070277TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC108450.058.595446
TATGCCG109050.058.27528848
GTATGCC111500.056.8396447
ATGCCGT113800.055.6934949
TCGTATG115800.054.63643345
GCACACG151800.053.7217611
ACGTCTG152050.053.65527715
CACACGT154450.053.04570812
CTCGTAT118850.052.2175844
GCCGTCT124050.050.87173551
TATCTCG122400.050.8428241
CACGTCT163700.049.8366114
ACACGTC165850.049.2747113
AGCACAC169000.048.45834410
TGCCGTC131600.048.05692750
CCTTTAT132050.048.01552237
TCTCGTA131200.047.4341243
TCCAGTC169500.047.1140825
GTCACAT140600.046.9118129
CAGTCAC146050.046.36459427
Produced by FastQC (version 0.11.2)