FastQCFastQC Report
Wed 1 Feb 2017
HGGHHBGX2_n01_9w-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGHHBGX2_n01_9w-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13158465
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG984490.7481799738799321RNA PCR Primer, Index 28 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG124950.061.6246746
CGTATGC127050.060.8214644
GTATGCC128850.059.94503445
ATGCCGT132150.058.16537547
TCGTATG133900.057.4012643
ACGTCTG172500.056.53364615
GCACACG173550.056.3108711
CACACGT177900.055.03112812
CTCGTAT137600.054.8298642
CACGTCT180900.053.9655414
GCCGTCT143550.053.71563349
AGCACAC189000.051.7439910
ACACGTC189200.051.70791613
AGTCACC154650.051.0146428
TCCAGTC189650.050.8930625
CTGAACT192350.050.86286519
ACTCCAG192400.050.5963223
TCTCGTA148550.050.53141841
CAGTCAC161800.050.03856727
CCAGTCA170850.049.4065326
Produced by FastQC (version 0.11.2)