FastQCFastQC Report
Wed 1 Feb 2017
HGGHHBGX2_n01_9w-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGHHBGX2_n01_9w-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14923609
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATTTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG773940.5186010970938731RNA PCR Primer, Index 35 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG105600.057.3023946
CGTATGC108000.055.9303644
GTATGCC111450.054.57044245
TCGTATG115200.052.31475443
ATGCCGT116350.051.9797647
ACGTCTG147650.051.65627715
GCACACG150250.051.08381711
CACACGT150150.050.95667612
CTCGTAT120700.049.27238542
TATCTCG120450.049.23129339
ACACGTC158550.048.3224213
CACGTCT160050.047.84782414
GCCGTCT127500.047.27330849
TGCCGTC130600.046.30848
AGCACAC169300.045.41725510
TCTCGTA131400.045.1811941
CGTCTGA174100.044.00701516
TCCAGTC175300.043.0358425
CTGAACT179400.042.7257219
ACTCCAG178250.042.67194723
Produced by FastQC (version 0.11.2)