HGGHHBGX2_n01_9w-19.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HGGHHBGX2_n01_9w-19.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14011051
Sequences flagged as poor quality	0
Sequence length	75
%GC	47

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG	68674	0.49014167459671654	RNA PCR Primer, Index 29 (100% over 43bp)
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTCGCGGG	21689	0.1547992366882399	No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACGAATTCAGACTGTGAAACTG	18965	0.13535744035190508	No Hit
CACGCTTTCACGGTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATT	18366	0.13108224358044232	No Hit
CTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGG	16583	0.1183565743925991	No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGATGTTATCC	15738	0.11232562068327352	No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT	14884	0.10623043196402611	No Hit
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAAGACCAGGAGCGT	14619	0.10433906778299501	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGTATGC	9615	60.70592	44
TATGCCG	9600	60.48017	46
GTATGCC	9985	58.422504	45
ATGCCGT	10200	56.958572	47
TCGTATG	10400	56.123787	43
GCACACG	13255	55.796032	11
ACGTCTG	13360	55.151352	15
CTCGTAT	10265	54.33968	42
CACACGT	13635	54.241028	12
CACGTCT	13950	52.892776	14
GCCGTCT	11105	51.884026	49
AGCACAC	14600	50.844933	10
AACTAAT	11375	50.61367	35
ACTCCAG	15085	48.63922	23
CAGTCAC	12655	48.52086	27
TCCAGTC	15135	48.43261	25
CTGAACT	15230	48.336075	19
AGTCACC	12345	48.28675	28
GAACTCC	15440	47.63397	21
CTAATCT	11680	47.57886	37