FastQCFastQC Report
Wed 1 Feb 2017
HGGHHBGX2_n01_9w-17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGHHBGX2_n01_9w-17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11079022
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1010560.9121382735768555RNA PCR Primer, Index 32 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG131850.062.0372646
CGTATGC134000.061.22016544
GTATGCC134800.060.85656445
TCGTATG136750.059.78669743
ATGCCGT138000.059.47442647
CTCGTAT137450.057.42443542
GCACACG176900.056.6497411
ACGTCTG177500.056.1856515
CACACGT178300.056.1081912
GCCGTCT148850.054.90891649
CACGTCT184600.054.08072714
ACACGTC186500.053.62273813
AGTCACC158550.052.85069328
TGCCGTC154950.052.81313348
TCTCGTA148550.052.73876641
TCCAGTC187150.052.62545425
AGCACAC191650.052.5417910
CCACTCA157350.052.0696133
CCAGTCA168200.051.8888626
ACTCCAG191000.051.80064823
Produced by FastQC (version 0.11.2)