FastQCFastQC Report
Wed 1 Feb 2017
HGGHHBGX2_n01_9w-14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGHHBGX2_n01_9w-14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9671099
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG304530.3148866535230381RNA PCR Primer, Index 34 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG67800.056.53523646
CGTATGC67250.056.17152844
GTATGCC71400.054.50166745
GCACACG142350.052.99873711
CACACGT144150.052.3132812
ACGTCTG144000.052.24691415
ACACGTC145100.051.94727313
ATGCCGT70400.050.72350747
CACGTCT150400.050.1383214
TCGTATG66250.049.72873743
AGCACAC155650.048.6033210
TCCAGTC149250.047.38154625
CGTCTGA159300.047.27313616
CTGAACT161600.046.36573819
ACTCCAG154950.046.35097523
CTCGTAT71300.046.30262442
AGTCACC125750.046.1157928
TGCCGTC78100.046.0311248
GAACTCC163050.045.2975421
CAGTCAC130700.045.1071327
Produced by FastQC (version 0.11.2)