FastQCFastQC Report
Wed 1 Feb 2017
HGGHHBGX2_n01_9w-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGHHBGX2_n01_9w-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12241254
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1497911.223657314846992RNA PCR Primer, Index 31 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG192400.063.15181446
CGTATGC193200.062.95993844
GTATGCC196700.061.89176645
ATGCCGT199550.060.76976847
TCGTATG201200.060.43941543
CACGATA200950.060.34170534
ACGTCTG235450.059.2645215
GCACACG236450.059.20377711
CTCGTAT204550.059.0103642
TATCTCG203750.058.98736639
CACACGT237800.058.8969312
ACACGTC242800.057.65587613
ACGATAT211150.057.6223635
GCCGTCT213000.056.78920749
CACGTCT246950.056.65857314
TCTCGTA213900.056.4465641
TGCCGTC215700.056.28375648
CCACGAT218950.055.77532233
AGCACAC252200.055.725110
AGTCACC223300.055.35241328
Produced by FastQC (version 0.11.2)