FastQCFastQC Report
Wed 1 Feb 2017
HGGHHBGX2_n01_9s-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGHHBGX2_n01_9s-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9122257
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG706100.7740408979926788RNA PCR Primer, Index 24 (100% over 43bp)
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTCGCGGG114510.12552814506322285No Hit
CACGCTTTCACGGTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATT104580.11464268108210501No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACGAATTCAGACTGTGAAACTG102110.1119350178360465No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT96090.10533577381123992No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG89500.062.40720746
CGTATGC89850.062.160744
ATGCCGT92300.060.40322547
GTATGCC93650.059.78708645
TCGTATG94550.059.0339443
ACGTCTG117600.058.40258415
GCACACG118400.058.15460611
GGTAGCA96600.057.81495734
CACACGT119750.057.35464512
AGTCACG100400.057.31126428
CTCGTAT96150.057.01046842
GTAGCAT98400.056.2318235
CACGTCT122000.056.21112414
GTCACGG101950.055.83086429
GCCGTCT102150.054.3772849
AGCACAC127550.054.22649810
CATCTCG100500.053.78739539
CTGAACT129200.053.32066319
TCCAGTC127950.053.2466825
ACTCCAG130450.052.46509623