Basic Statistics
Measure | Value |
---|---|
Filename | HGGHHBGX2_n01_9s-8.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 9122257 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 47 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG | 70610 | 0.7740408979926788 | RNA PCR Primer, Index 24 (100% over 43bp) |
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTCGCGGG | 11451 | 0.12552814506322285 | No Hit |
CACGCTTTCACGGTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATT | 10458 | 0.11464268108210501 | No Hit |
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACGAATTCAGACTGTGAAACTG | 10211 | 0.1119350178360465 | No Hit |
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT | 9609 | 0.10533577381123992 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATGCCG | 8950 | 0.0 | 62.407207 | 46 |
CGTATGC | 8985 | 0.0 | 62.1607 | 44 |
ATGCCGT | 9230 | 0.0 | 60.403225 | 47 |
GTATGCC | 9365 | 0.0 | 59.787086 | 45 |
TCGTATG | 9455 | 0.0 | 59.03394 | 43 |
ACGTCTG | 11760 | 0.0 | 58.402584 | 15 |
GCACACG | 11840 | 0.0 | 58.154606 | 11 |
GGTAGCA | 9660 | 0.0 | 57.814957 | 34 |
CACACGT | 11975 | 0.0 | 57.354645 | 12 |
AGTCACG | 10040 | 0.0 | 57.311264 | 28 |
CTCGTAT | 9615 | 0.0 | 57.010468 | 42 |
GTAGCAT | 9840 | 0.0 | 56.23182 | 35 |
CACGTCT | 12200 | 0.0 | 56.211124 | 14 |
GTCACGG | 10195 | 0.0 | 55.830864 | 29 |
GCCGTCT | 10215 | 0.0 | 54.37728 | 49 |
AGCACAC | 12755 | 0.0 | 54.226498 | 10 |
CATCTCG | 10050 | 0.0 | 53.787395 | 39 |
CTGAACT | 12920 | 0.0 | 53.320663 | 19 |
TCCAGTC | 12795 | 0.0 | 53.24668 | 25 |
ACTCCAG | 13045 | 0.0 | 52.465096 | 23 |