FastQCFastQC Report
Wed 1 Feb 2017
HGGHHBGX2_n01_9s-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGHHBGX2_n01_9s-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11102112
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA2463992.219388527155914TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG302800.065.58292448
CGTATGC305300.065.26778446
GTATGCC308350.064.4578647
TCGTATG309750.064.2826745
ATGCCGT309000.064.2258449
CTCGTAT303750.063.6904844
GCCGTCT315200.062.78151351
AGTCACG324600.062.71023628
GTCACGT323650.062.34007629
TCACGTG322800.062.32256330
ACGTCTG378050.062.2472115
GCACACG380000.062.1200911
CACGTGA323800.062.06645231
CACACGT382000.061.77679412
CACGTCT385850.061.06012314
TCTCGTA315650.060.7101243
AACGATC314300.060.4970238
ACGTGAA331600.060.46140732
ACACGTC390900.060.36116813
AGCACAC392250.060.24163410
Produced by FastQC (version 0.11.2)