FastQCFastQC Report
Wed 1 Feb 2017
HGGHHBGX2_n01_9s-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGHHBGX2_n01_9s-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11357257
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA705880.621523313243682TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG97500.059.20026848
CGTATGC98450.058.76504546
ATGCCGT102300.056.49297749
GTATGCC103650.055.75349847
GGCCTTA102750.055.59653536
TCGTATG105250.054.89989545
GCACACG142600.054.23403511
ACGTCTG142700.053.97847715
GCCTTAT106350.053.39020537
AGTCACG112700.053.1678328
CACACGT145150.053.114912
CTCGTAT108300.052.4300444
GTCACGT113400.052.1706329
TATCTCG108400.051.61540241
TCACGTG113950.051.5252630
ACGTGGC114550.050.89432
CACGTGG115250.050.82411631
ACACGTC152500.050.5103613
GCCGTCT114400.050.30996751
CACGTCT153850.050.0662614
Produced by FastQC (version 0.11.2)