FastQCFastQC Report
Wed 1 Feb 2017
HGGHHBGX2_n01_9s-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGHHBGX2_n01_9s-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10066037
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG821610.8162199284584389RNA PCR Primer, Index 26 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC107700.061.4028344
TATGCCG109250.060.4405446
GTATGCC112450.059.02503245
TCGTATG111800.058.1013443
ATGCCGT113450.057.6874747
CTCGTAT114350.056.17077642
ACGTCTG137050.054.99621615
GCACACG139050.054.45326611
GCCGTCT119400.053.9483449
CACACGT141100.053.5159812
TGCCGTC124400.052.38858848
ACACGTC144050.052.37265413
TCTCGTA123150.052.0726541
CACGTCT145450.051.98636214
CATCTCG124850.051.77857239
TCCAGTC149800.050.06149325
GTCACAT138700.050.04087429
CATGAGC140500.049.37522533
AGCACAC154450.049.22506310
CAGTCAC142550.049.1723827
Produced by FastQC (version 0.11.2)