FastQCFastQC Report
Wed 1 Feb 2017
HGGHHBGX2_n01_9s-19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGHHBGX2_n01_9s-19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11114235
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA937190.8432339247820475TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG123250.062.6740148
CGTATGC124700.061.94329546
GTATGCC127200.060.91735547
TCGTATG127350.059.9746545
ATGCCGT128050.059.7335349
AGTCACG140000.059.21242528
ACGTCTG170050.058.6065715
GTCACGT140450.058.50690529
CGTTTCG139550.058.2162633
GCACACG171300.058.03794511
CTCGTAT129950.057.65921444
GCCGTCT130550.057.5081951
CACACGT175200.056.8050712
CACGTCT177350.056.1745314
CACGTTT145250.056.14544731
GTTTCGG148850.054.76486234
AGCACAC183850.054.1697210
ACACGTC184950.053.79207213
TCCAGTC182600.053.69089525
ACTCCAG185050.053.38968723
Produced by FastQC (version 0.11.2)