FastQCFastQC Report
Wed 1 Feb 2017
HGGHHBGX2_n01_9s-17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGHHBGX2_n01_9s-17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9291220
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA668420.7194103680679179TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG89300.060.57803348
CGTATGC90800.060.14366546
GTATGCC91200.059.57982647
ATGCCGT94600.057.2950849
TCGTATG95150.057.1027845
GCACACG118200.056.56100511
AGTCACG101150.055.99940528
CACACGT119150.055.99422512
CGAGTGG99150.055.7720933
ACGTCTG119350.055.75527215
CTCGTAT95900.055.64808344
ACGAGTG100400.055.1464432
CACGAGT102700.054.04577331
GTCACGA104250.054.03638529
CACGTCT123950.053.79742414
GCCGTCT101150.053.48517651
TATCTCG99850.053.27364741
AGCACAC130150.051.6327910
TCCAGTC128100.051.19225
ACACGTC130550.051.18419613
Produced by FastQC (version 0.11.2)