FastQCFastQC Report
Wed 1 Feb 2017
HGGHHBGX2_n01_9s-14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGHHBGX2_n01_9s-14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10968941
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA669420.6102868089089002TruSeq Adapter, Index 25 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC89050.060.51445846
TATGCCG89550.059.8331248
GTATGCC91850.058.37161647
TCGTATG96100.056.2527545
ATGCCGT97150.055.1894349
CTCGTAT96100.054.88836344
ACGTCTG135150.054.74919515
GCACACG135950.054.70738211
CACACGT137250.054.1640712
GCCGTCT102850.052.1350851
CACGTCT143450.051.67807414
TATCTCG101350.051.60025441
AGCACAC149650.049.8604510
CACACTG110600.049.74940531
TCCAGTC141900.049.44689625
ACACGTC152350.048.7279513
ACTCCAG148050.048.16134323
TCTCGTA108950.048.06564743
TATATCT110050.047.9911939
TGATATA114450.047.71352436
Produced by FastQC (version 0.11.2)