FastQCFastQC Report
Wed 1 Feb 2017
HGGHHBGX2_n01_9s-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGHHBGX2_n01_9s-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11737819
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1270181.0821260746992265TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG160850.063.21018248
CGTATGC161800.063.1791446
GTACGTA162050.063.0337235
CGTACGT163700.062.33595334
TACGTAA164150.061.765536
CCGTACG165650.061.7476633
GTATGCC165850.061.3872347
ATGCCGT166700.060.89028549
TCGTATG168200.060.6897745
ACGTAAT169850.059.3677437
GCACACG200250.059.22434211
ACGTCTG199900.059.17323315
CTCGTAT169050.058.83317644
CACACGT205100.057.8745712
ACCGTAC178600.057.347732
GCCGTCT177100.057.33600251
CACGTCT208600.056.78800214
ACACGTC215050.055.1486913
AGCACAC215800.055.06844710
GTCACCG190150.054.26321429
Produced by FastQC (version 0.11.2)