FastQCFastQC Report
Wed 1 Feb 2017
HGGHHBGX2_n01_4w-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGHHBGX2_n01_4w-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12459406
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG275790.2213508412840869TruSeq Adapter, Index 16 (98% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTCC45200.051.74585733
TATGCCG46200.049.955846
CGTATGC47000.049.2507144
GTATGCC50750.045.6794745
GCACACG66950.044.5707411
TCGTATG52850.043.4074743
ACGTCTG69650.043.0897315
CACACGT70200.042.7041912
ATGCCGT54550.042.31073447
CACGTCT78400.038.1489114
CTCGTAT58950.037.68655442
AGCACAC81450.037.0596510
ACACGTC80750.036.95384613
CACCCGT64450.036.50444831
CCGTCCA64900.036.19832634
GCCGTCT65500.036.02893449
TCACCCG66900.035.63153530
CGTCCAT67850.033.81085635
CATCTCG66550.033.27934639
GTCACCC72150.033.2297929
Produced by FastQC (version 0.11.2)