FastQCFastQC Report
Wed 1 Feb 2017
HGGHHBGX2_n01_4w-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGHHBGX2_n01_4w-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16510037
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG764940.4633181621579649TruSeq Adapter, Index 10 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC112950.055.92196744
TATGCCG113900.055.39697646
GTATGCC120550.052.3393245
ATGCCGT120800.052.03504647
GCACACG165700.051.23407411
CACACGT166700.050.9063512
ACGTCTG167200.050.6709715
TCGTATG125000.050.2002243
ACACGTC175150.048.50977713
CTCGTAT129350.048.00469642
TATCTCG131800.047.29556339
CACGTCT179550.047.2818714
TGCCGTC133400.047.0171748
CACTAGC142000.046.54640231
GCCGTCT137550.045.375549
AGCACAC188900.045.1789610
TAGCTTA144850.044.8687534
CGTCTGA190150.044.7199116
TCACTAG149150.044.6852530
TCTCGTA144000.043.00123641
Produced by FastQC (version 0.11.2)