FastQCFastQC Report
Wed 1 Feb 2017
HGGHHBGX2_n01_4w-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGHHBGX2_n01_4w-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13671731
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1097700.8028975994334587TruSeq Adapter, Index 11 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG147850.061.0402946
CGTATGC149350.060.4725544
GTATGCC153700.058.69372645
ATGCCGT156600.057.50086647
TCGTATG159350.056.54770343
ACGTCTG199150.055.0142215
GCACACG201400.054.67426711
CACACGT201050.054.666712
AGTCACG169700.054.66138528
CTCGTAT163700.054.30588542
CACGGCT169900.053.4799331
GTCACGG173750.053.0497429
GCCGTCT170700.052.77298749
ACGGCTA171600.052.76919632
CGGCTAC170850.052.7383633
CACGTCT208800.052.52141614
GCTACAT170400.052.3115735
GGCTACA171200.052.26861634
CATCTCG170600.051.84658439
ACACGTC212100.051.80240613
Produced by FastQC (version 0.11.2)