FastQCFastQC Report
Wed 1 Feb 2017
HGGHHBGX2_n01_4w-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGHHBGX2_n01_4w-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12016818
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA780560.6495563134933058TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC102400.063.09845733
TATGCCG106950.060.485648
CCGCACA106300.059.87429436
CGCACAT106250.059.70716537
TCCGCAC107600.059.6636235
CGTATGC108950.059.33964546
GTCCGCA109050.059.0605934
GTATGCC112350.057.67054447
ATGCCGT114450.056.554949
ACGTCCG114450.056.4249232
TCGTATG115800.055.916345
GCACACG144100.055.4908411
ACGTCTG144650.055.30301315
AGTCACG120550.055.19972228
CACACGT146250.054.72248512
GTCACGT120500.054.59304429
CTCGTAT118850.053.6107944
ACACGTC152550.052.4849513
CACGTCT154150.051.8498114
GCCGTCT125050.051.7363851