Basic Statistics
Measure | Value |
---|---|
Filename | HGGHHBGX2_n01_4w-2.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 12016818 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA | 78056 | 0.6495563134933058 | TruSeq Adapter, Index 18 (97% over 40bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTCCGC | 10240 | 0.0 | 63.098457 | 33 |
TATGCCG | 10695 | 0.0 | 60.4856 | 48 |
CCGCACA | 10630 | 0.0 | 59.874294 | 36 |
CGCACAT | 10625 | 0.0 | 59.707165 | 37 |
TCCGCAC | 10760 | 0.0 | 59.66362 | 35 |
CGTATGC | 10895 | 0.0 | 59.339645 | 46 |
GTCCGCA | 10905 | 0.0 | 59.06059 | 34 |
GTATGCC | 11235 | 0.0 | 57.670544 | 47 |
ATGCCGT | 11445 | 0.0 | 56.5549 | 49 |
ACGTCCG | 11445 | 0.0 | 56.42492 | 32 |
TCGTATG | 11580 | 0.0 | 55.9163 | 45 |
GCACACG | 14410 | 0.0 | 55.49084 | 11 |
ACGTCTG | 14465 | 0.0 | 55.303013 | 15 |
AGTCACG | 12055 | 0.0 | 55.199722 | 28 |
CACACGT | 14625 | 0.0 | 54.722485 | 12 |
GTCACGT | 12050 | 0.0 | 54.593044 | 29 |
CTCGTAT | 11885 | 0.0 | 53.61079 | 44 |
ACACGTC | 15255 | 0.0 | 52.48495 | 13 |
CACGTCT | 15415 | 0.0 | 51.84981 | 14 |
GCCGTCT | 12505 | 0.0 | 51.73638 | 51 |