FastQCFastQC Report
Wed 1 Feb 2017
HGGHHBGX2_n01_4w-19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGHHBGX2_n01_4w-19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14700124
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA923660.6283348358149904TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG125750.059.72865748
CGTATGC127250.059.4539446
GTATGCC132100.057.14500847
ATGCCGT133350.056.4033249
TCGTATG137000.054.86832445
GCACACG172700.054.62976511
ACGTCTG174700.053.96376415
CACACGT176500.053.55132312
CTCGTAT137800.052.79720344
CACGTCT183900.051.3952314
ACACGTC185400.051.01766613
GCCGTCT149050.050.5576651
AGCACAC189800.049.88982410
TGCCGTC156450.048.0764550
TCTCGTA151350.048.04776443
ACTCCAG198000.047.59751523
TCCAGTC197450.047.50233525
CAGTCAC170750.047.09202627
CTGAACT202700.046.47780219
GTCACAG169650.045.9746529
Produced by FastQC (version 0.11.2)