FastQCFastQC Report
Wed 1 Feb 2017
HGGHHBGX2_n01_4w-17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGHHBGX2_n01_4w-17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13207462
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA653550.4948339052574976TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG89050.056.7577248
CGTATGC90650.055.79293446
GTATGCC93100.054.3996447
ATGCCGT95000.053.09616549
TCGTATG97550.051.84300245
GCACACG115450.051.03434811
ACGTCTG116100.050.7476715
CACACGT117350.050.3254512
CTCGTAT102150.048.79848544
ACACGTC123350.047.87751813
CACGTCT127450.046.2826614
TGCCGTC110400.045.34706550
GCCGTCT110350.045.1188551
TCTCGTA113100.044.3179743
AGCACAC135750.043.68221710
GTCACAT123350.043.1514529
CGTCTGA137950.042.81102816
CAGTCAC128500.042.41498627
ATGTCAG126300.041.95189334
TCCAGTC142650.041.13324425
Produced by FastQC (version 0.11.2)