FastQCFastQC Report
Wed 1 Feb 2017
HGGHHBGX2_n01_4w-14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGHHBGX2_n01_4w-14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12261618
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG734860.5993173168500274RNA PCR Primer, Index 17 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC97900.059.3401444
TATGCCG99050.058.30575646
GTATGCC102900.056.32242245
TCGTATG105550.054.90902743
GCACACG139650.054.7890611
ATGCCGT105650.054.5671947
ACGTCTG141500.053.87682315
TCACGTA109550.053.56456430
CACACGT146500.052.25079712
AGTCACG114700.052.12239528
CTCGTAT108900.051.7297542
ACGTAGA112200.051.7152732
GTCACGT114950.051.2285429
CACGTCT151300.050.5015314
ACACGTC152850.049.9900213
GCCGTCT116550.049.5835749
CGTAGAG118150.049.51967633
AGCACAC156850.048.9129110
CACGTAG121200.048.13119531
TGCCGTC121250.047.71711348
Produced by FastQC (version 0.11.2)