FastQCFastQC Report
Wed 1 Feb 2017
HGGHHBGX2_n01_4w-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGHHBGX2_n01_4w-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13784597
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1048170.7603921971748612TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC134900.062.1186946
TATGCCG135750.061.60556848
GTATGCC139200.060.30002247
ATGCCGT139000.060.0423649
TCGTATG142000.059.03576745
GCACACG174550.058.42061211
ACGTCTG174400.058.2539115
CACACGT176050.057.88429612
CGTATCT143450.057.45052339
TCCGTAT145550.057.33286337
CTCGTAT145750.056.85368344
TTCCGTA148400.056.5808936
CCGTATC147400.055.9573838
CACGTCT183750.055.3270414
GTTCCGT152350.055.20468535
GCCGTCT151100.055.03189551
TATCTCG150450.054.8921641
AGCACAC188500.054.2069810
ACACGTC191300.053.2700813
TCCAGTC192550.052.34942625
Produced by FastQC (version 0.11.2)