FastQCFastQC Report
Wed 1 Feb 2017
HGGHHBGX2_n01_4s-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGHHBGX2_n01_4s-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12107135
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG689780.5697301632467136TruSeq Adapter, Index 7 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG92300.059.0572946
CGTATGC92250.059.04677644
GTATGCC96250.056.80933845
ATGCCGT99200.054.8130847
TCGTATG100300.054.03213543
GCACACG124500.053.69595311
ACGTCTG124850.053.351815
CACACGT127450.052.50699612
CTCGTAT102400.051.8443242
GCCGTCT108800.049.60019749
CACGTCT135050.049.37314614
ACACGTC136450.048.89204813
CATCTCG109900.048.36883539
AGCACAC142350.046.98697310
TGCCGTC116600.046.8409448
AGTCACC121900.046.7203228
TCTCGTA115500.045.96357341
TCCAGTC143450.045.90431225
CAGTCAC126000.045.7744227
CTGAACT149800.044.62953619
Produced by FastQC (version 0.11.2)