FastQCFastQC Report
Wed 1 Feb 2017
HGGHHBGX2_n01_4s-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGHHBGX2_n01_4s-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24920543
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG4954291.9880345303872393TruSeq Adapter, Index 1 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG619600.064.43146546
CGTATGC624150.064.053844
GTATGCC629500.063.46035845
ATGCCGT634450.062.89188847
TCGTATG636750.062.6455743
CTCGTAT633700.061.26964642
TGCCGTC655800.060.7505748
ACGTCTG706100.060.67273315
GCACACG709550.060.48456611
GCCGTCT658300.060.43306449
CACACGT715250.060.00254412
TCTCGTA645550.059.57850641
ACACGTC720850.059.54657413
CACGTCT729400.058.75824414
GTCACAT687000.058.38899229
CATCACG687200.058.22175633
AGCACAC741700.057.988710
CGTCTGA740050.057.93223616
TCACGAT682700.057.56435835
CACGATC665200.057.414436
Produced by FastQC (version 0.11.2)