FastQCFastQC Report
Wed 1 Feb 2017
HGGHHBGX2_n01_4s-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGHHBGX2_n01_4s-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19813328
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1096850.5535920063504728TruSeq Adapter, Index 2 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG147300.056.65449546
CGTATGC148850.056.13222544
GTATGCC153500.054.36452545
ATGCCGT155750.053.40546847
TCGTATG160350.051.86961443
GCACACG193100.051.1988311
ACGTCTG196500.050.2774615
CACACGT197700.050.00718712
CTCGTAT167350.049.2046342
TATCTCG169250.048.4075639
ACACGTC205500.048.14291413
CGATGTA175750.047.83539234
CCGATGT178300.047.4029133
TGCCGTC177000.047.07248
GCCGTCT179250.046.4432349
CACGTCT215800.045.84485214
AGCACAC218350.045.452110
TCTCGTA181150.045.32258241
CGTCTGA225350.043.85664416
TCCAGTC231150.042.38278625
Produced by FastQC (version 0.11.2)