FastQCFastQC Report
Wed 1 Feb 2017
HGGHHBGX2_n01_4s-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGHHBGX2_n01_4s-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12559535
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG957910.7626954341860587TruSeq Adapter, Index 9 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG128200.061.031646
CGTATGC130400.059.97331644
GTATGCC133650.058.43773345
ATGCCGT135450.057.6122847
TCGTATG138600.056.2262243
ACGTCTG160950.055.53177615
AGTCACG144250.055.33794428
GCACACG164950.054.2899311
CACACGT165850.054.07872812
CTCGTAT140700.054.03872742
GTCACGA148600.053.25374229
ACACGTC168000.053.24312613
TGCCGTC147650.052.8521148
GCCGTCT150000.052.1645849
CACGTCT171850.051.92945514
TCTCGTA152600.049.82449741
CGTCTGA181750.049.2538516
AGCACAC184000.048.9314210
CGATCAG160700.048.836333
TCCAGTC186350.047.8151325
Produced by FastQC (version 0.11.2)