FastQCFastQC Report
Wed 1 Feb 2017
HGGHHBGX2_n01_4s-19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGHHBGX2_n01_4s-19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16491393
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1453960.8816477783289743TruSeq Adapter, Index 3 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG190200.061.79632646
CGTATGC193100.060.88372844
GTATGCC196050.059.9329545
ATGCCGT199200.058.92022747
TCGTATG201950.058.09570743
TAGGCAT205900.056.6122635
CACTTAG212400.056.309731
ACTTAGG211900.056.24720832
TTAGGCA211500.055.9947534
CACACGT247700.055.623312
CTCGTAT207100.055.46685842
GCACACG248550.055.2663611
ACGTCTG248050.055.23835815
TGCCGTC215900.054.2034148
GCCGTCT215450.054.1906549
ACACGTC259200.052.92903513
CATCTCG215000.052.7394939
CACGTCT260650.052.6081214
GGCATCT219300.051.89430637
TCACTTA232700.051.84201430
Produced by FastQC (version 0.11.2)