FastQCFastQC Report
Wed 1 Feb 2017
HGGHHBGX2_n01_4s-17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGHHBGX2_n01_4s-17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12911266
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG810520.6277618321859375TruSeq Adapter, Index 5 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC108750.059.76302744
TATGCCG110900.058.4836146
GTATGCC113650.057.2171545
ATGCCGT116550.055.67939447
TCGTATG118700.054.57966243
ACGTCTG143550.053.515915
GCACACG145150.052.92619711
CTCGTAT118700.051.90549542
CACACGT149250.051.54121412
GCCGTCT129350.050.0648149
CACGTCT155100.049.6861714
CACAGTG133550.049.5946633
ACACGTC156450.049.1692313
CACACAG136550.048.45454431
TGCCGTC133900.048.2071248
ACACAGT138350.047.7742532
AGCACAC161850.047.74205810
TCTCGTA130250.047.30201341
CAGTCAC148850.046.1876627
GTCACAC144850.045.84459329
Produced by FastQC (version 0.11.2)