FastQCFastQC Report
Wed 1 Feb 2017
HGGHHBGX2_n01_4s-14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGHHBGX2_n01_4s-14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12563159
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG910250.7245391067644691TruSeq Adapter, Index 8 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG121700.061.08873446
CGTATGC122800.060.5955444
GTATGCC128950.058.054545
ATGCCGT131700.056.24226847
TCGTATG131450.056.21509643
GCACACG146200.055.1894611
ACGTCTG148000.054.5646415
CACACGT148950.054.21684312
CTCGTAT136000.053.85112442
GCCGTCT138300.052.81198549
TGCCGTC143000.051.7263948
CACGTCT156300.051.66709514
ACACGTC156350.051.62891813
GTCACAC153150.050.27622229
TCTCGTA146350.050.066141
AGCACAC165000.049.11008510
CAGTCAC161000.047.9949427
CGTCTGA173100.046.7528116
TCCAGTC171350.046.52511225
ACTCCAG173250.046.43338423
Produced by FastQC (version 0.11.2)