FastQCFastQC Report
Wed 1 Feb 2017
HGGHHBGX2_n01_4s-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGHHBGX2_n01_4s-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14929543
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1734441.1617502290592552TruSeq Adapter, Index 4 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG213300.064.3705346
CGTATGC215450.063.9341844
GTATGCC219400.062.7053645
ATGCCGT221250.062.124547
ACTGACC222350.061.5763732
TCGTATG223800.061.5335943
GCACACG242550.060.9133611
ACGTCTG242950.060.6132715
CACACGT245550.060.0848612
CTCGTAT221800.059.92533542
GCCGTCT234450.058.5551549
CACTGAC235100.058.44236831
CACGTCT255050.057.77824814
AGCACAC257850.057.41935310
ACACGTC261750.056.39231513
TCTCGTA235850.056.1366541
AGTCACT248650.055.89651528
CAGTCAC252450.055.2730327
TCCAGTC265850.055.10832225
ACTCCAG267650.055.0089223
Produced by FastQC (version 0.11.2)