FastQCFastQC Report
Wed 12 May 2021
HGGF2BGXJ_n02_ReSeq_Sp20_Neg_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGF2BGXJ_n02_ReSeq_Sp20_Neg_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112702
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGCTTAATTGC18921.67876346471225No Hit
GCTCTATCCCCAGCACGACAGGGTTTAACAAGATTACCCAAACCTCTCGG13801.2244680662277512No Hit
AAATAAGGATTGACAGATTGAGAGCTCTTTCTTGATCTTTTGGATGGTGG11200.9937711841848414No Hit
GACGTAATCGGCACGAGCTGATGACTCGTGCCTACTAGGCATTCCTCGTT8980.7967915387482032No Hit
CTACTAGGCATTCCTCGTTGAAGAGCAATAATTGCAATGCTCTATCCCCA8260.7329062483363206No Hit
AGCGAAAGCAGGTCCAGACAAAATAAGGATTGACAGATTGAGAGCTCTTT7700.6832176891270785No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGCTTAATTGCGAT7420.6583734095224575No Hit
GCTTAATTGCGATAACGAACGAGACCTCGGCCCTTAAATAGCCCGGTCCG7380.6548242267217973No Hit
GGTCCAGACAAAATAAGGATTGACAGATTGAGAGCTCTTTCTTGATCTTT6920.6140086245142057No Hit
ATATTGTACCACTTCAGAATCTTTCGGTTTTTCCCTTGATGCTTCTCTCT6700.5944881191105748No Hit
TTCTTGATCTTTTGGATGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTG6690.5936008234104098No Hit
GTATAGACTCTGTGCAAAGTACCTTAAAAACATCAACTCAAGAGAAGGGA6430.5705311352061188No Hit
ATCCCCAGCACGACAGGGTTTAACAAGATTACCCAAACCTCTCGGTTAAG6280.5572216997036432No Hit
GTCAGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCT5970.5297155329985271No Hit
AGCGAAAGCAGGGACGTAATCGGCACGAGCTGATGACTCGTGCCTACTAG5450.4835761565899451No Hit
TAAATAGCCCGGTCCGCATTTGCGGGCCGCTGGCTTCTTAAGGGGACTAT5280.46849212968713955No Hit
ACCCAAACCTCTCGGTTAAGGTGATGTACTCGCTGGCCCTGTCAGTGTAG5240.4649429468864794No Hit
GCCCAGAACATCTAAGGGCATCACAGACCTGTTATTGCCGCGCACTTCCA5030.44630973718301364No Hit
AAATAGCCCGGTCCGCATTTGCGGGCCGCTGGCTTCTTAAGGGGACTATC4880.43300030168053805No Hit
TTTGGATGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGC4780.4241273446788877No Hit
CGGTTAAGGTGATGTACTCGCTGGCCCTGTCAGTGTAGCGCGCGTGCGGC4700.41702897907756736No Hit
TTCTTAGTTGGTGGAGTGATTTGTCTGCTTAATTGCGATAACGAACGAGA4610.4090433177760821No Hit
GGGTTTAACAAGATTACCCAAACCTCTCGGTTAAGGTGATGTACTCGCTG4390.38952281237245123No Hit
CCCTTAAATAGCCCGGTCCGCATTTGCGGGCCGCTGGCTTCTTAAGGGGA4260.3779879682703058No Hit
CTTTTGGATGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCT4210.37355148976948055No Hit
GATTGAGAGCTCTTTCTTGATCTTTTGGATGGTGGTGCATGGCCGTTCTT4160.36911501126865537No Hit
TTGCAATGCTCTATCCCCAGCACGACAGGGTTTAACAAGATTACCCAAAC4000.35491828006601484No Hit
TGTCAGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACC3960.35136909726535465No Hit
GTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTA3940.3495945058650246No Hit
GACAGATTGAGAGCTCTTTCTTGATCTTTTGGATGGTGGTGCATGGCCGT3630.3220883391599084No Hit
CTGATGACTCGTGCCTACTAGGCATTCCTCGTTGAAGAGCAATAATTGCA3420.30345512945644265No Hit
CCCCAGCACGACAGGGTTTAACAAGATTACCCAAACCTCTCGGTTAAGGT3340.29635676385512233No Hit
GTTATTGCCGCGCACTTCCATCGGCTTGAGCCGATAGTCCCCTTAAGAAG3260.28925839825380206No Hit
ATTGAGAGCTCTTTCTTGATCTTTTGGATGGTGGTGCATGGCCGTTCTTA3250.288371102553637No Hit
AGATTACCCAAACCTCTCGGTTAAGGTGATGTACTCGCTGGCCCTGTCAG3160.2803854412521517No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCCGCGCACTTCCATCGGCTTG3130.27772355415165656No Hit
ATTTTGTATAGACTCTGTGCAAAGTACCTTAAAAACATCAACTCAAGAGA3100.2750616670511615No Hit
GCAATGCTCTATCCCCAGCACGACAGGGTTTAACAAGATTACCCAAACCT3050.2706251885503363No Hit
TCACAGACCTGTTATTGCCGCGCACTTCCATCGGCTTGAGCCGATAGTCC2950.2617522315486859No Hit
CTATTAGTCTACCACCAAATGCCTCAAAAAACAGCCCTAATCTCAGCAGG2940.2608649358485209No Hit
GTACTCGCTGGCCCTGTCAGTGTAGCGCGCGTGCGGCCCAGAACATCTAA2870.2546538659473656No Hit
GATTTGTCTGCTTAATTGCGATAACGAACGAGACCTCGGCCCTTAAATAG2770.24578090894571528No Hit
GATGACTCGTGCCTACTAGGCATTCCTCGTTGAAGAGCAATAATTGCAAT2720.24134443044489007No Hit
CTTAAGGGGACTATCGGCTCAAGCCGATGGAAGTGCGCGGCAATAACAGG2710.24045713474472502No Hit
GTCCAGACAAAATAAGGATTGACAGATTGAGAGCTCTTTCTTGATCTTTT2650.2351333605437348No Hit
AAGCAGGTCCAGACAAAATAAGGATTGACAGATTGAGAGCTCTTTCTTGA2620.2324714734432397No Hit
GGTTTAACAAGATTACCCAAACCTCTCGGTTAAGGTGATGTACTCGCTGG2590.2298095863427446No Hit
GCGAAAGCAGGTCCAGACAAAATAAGGATTGACAGATTGAGAGCTCTTTC2590.2298095863427446No Hit
GCAGGGACGTAATCGGCACGAGCTGATGACTCGTGCCTACTAGGCATTCC2580.22892229064257955No Hit
GCCCTTAAATAGCCCGGTCCGCATTTGCGGGCCGCTGGCTTCTTAAGGGG2530.22448581214175437No Hit
GTCTATGAATATGCTATCCCTACAAATTCAGATTGGATGAAGGATCCTTT2520.22359851644158935No Hit
GTTTAACAAGATTACCCAAACCTCTCGGTTAAGGTGATGTACTCGCTGGC2500.22182392504125925No Hit
AAGCAGGGACGTAATCGGCACGAGCTGATGACTCGTGCCTACTAGGCATT2470.2191620379407641No Hit
GTTCTTAGTTGGTGGAGTGATTTGTCTGCTTAATTGCGATAACGAACGAG2460.2182747422405991No Hit
GGATTGACAGATTGAGAGCTCTTTCTTGATCTTTTGGATGGTGGTGCATG2460.2182747422405991No Hit
CTTCATAGCAGATATTGGTAGATGCTTTTTAGCTAAGCTGAATTGGCTCA2440.21650015084026902No Hit
CTCGTGCCTACTAGGCATTCCTCGTTGAAGAGCAATAATTGCAATGCTCT2390.21206367233944384No Hit
GATTGACAGATTGAGAGCTCTTTCTTGATCTTTTGGATGGTGGTGCATGG2330.20673989813845364No Hit
GTTAAGGTGATGTACTCGCTGGCCCTGTCAGTGTAGCGCGCGTGCGGCCC2330.20673989813845364No Hit
CAATAATTGCAATGCTCTATCCCCAGCACGACAGGGTTTAACAAGATTAC2280.20230341963762846No Hit
GCTGATGACTCGTGCCTACTAGGCATTCCTCGTTGAAGAGCAATAATTGC2230.19786694113680328No Hit
AAGCAGGAGTGTGTGCTCCATCAGCCTGAAAAATAACAGGGCTGAGGATA2220.1969796454366382No Hit
GTGATGTACTCGCTGGCCCTGTCAGTGTAGCGCGCGTGCGGCCCAGAACA2180.19343046263597807No Hit
AGCAGGTCCAGACAAAATAAGGATTGACAGATTGAGAGCTCTTTCTTGAT2170.19254316693581303No Hit
CAGATTGAGAGCTCTTTCTTGATCTTTTGGATGGTGGTGCATGGCCGTTC2160.19165587123564798No Hit
GCAATAATTGCAATGCTCTATCCCCAGCACGACAGGGTTTAACAAGATTA2120.18810668843498785No Hit
CAAAATAAGGATTGACAGATTGAGAGCTCTTTCTTGATCTTTTGGATGGT2110.1872193927348228No Hit
ACTAGGCATTCCTCGTTGAAGAGCAATAATTGCAATGCTCTATCCCCAGC2100.18633209703465778No Hit
ATTTAAGGGCCGAGGTCTCGTTCGTTATCGCAATTAAGCAGACAAATCAC2080.18455750563432768No Hit
CTCTATCCCCAGCACGACAGGGTTTAACAAGATTACCCAAACCTCTCGGT2040.18100832283366755No Hit
CCTCTCGGTTAAGGTGATGTACTCGCTGGCCCTGTCAGTGTAGCGCGCGT2040.18100832283366755No Hit
TCTAAGGGCATCACAGACCTGTTATTGCCGCGCACTTCCATCGGCTTGAG2030.1801210271335025No Hit
CTATCGGCTCAAGCCGATGGAAGTGCGCGGCAATAACAGGTCTGTGATGC2030.1801210271335025No Hit
AGGATTGACAGATTGAGAGCTCTTTCTTGATCTTTTGGATGGTGGTGCAT2030.1801210271335025No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGCTTAATTGCGATAACGAAC2000.17745914003300742No Hit
TAATTGCAATGCTCTATCCCCAGCACGACAGGGTTTAACAAGATTACCCA1990.17657184433284234No Hit
AGCTGATGACTCGTGCCTACTAGGCATTCCTCGTTGAAGAGCAATAATTG1990.17657184433284234No Hit
GTGGAGTGATTTGTCTGCTTAATTGCGATAACGAACGAGACCTCGGCCCT1980.17568454863267732No Hit
GATGTACTCGCTGGCCCTGTCAGTGTAGCGCGCGTGCGGCCCAGAACATC1940.17213536583201716No Hit
AGCAGGGACGTAATCGGCACGAGCTGATGACTCGTGCCTACTAGGCATTC1940.17213536583201716No Hit
AGGCATTCCTCGTTGAAGAGCAATAATTGCAATGCTCTATCCCCAGCACG1920.1703607744316871No Hit
GAACGAGACCTCGGCCCTTAAATAGCCCGGTCCGCATTTGCGGGCCGCTG1920.1703607744316871No Hit
ATCTAAGGGCATCACAGACCTGTTATTGCCGCGCACTTCCATCGGCTTGA1920.1703607744316871No Hit
GACAAAATAAGGATTGACAGATTGAGAGCTCTTTCTTGATCTTTTGGATG1900.16858618303135703No Hit
TAGTTGGTGGAGTGATTTGTCTGCTTAATTGCGATAACGAACGAGACCTC1900.16858618303135703No Hit
TGCCTACTAGGCATTCCTCGTTGAAGAGCAATAATTGCAATGCTCTATCC1890.16769888733119198No Hit
TCGTTGAAGAGCAATAATTGCAATGCTCTATCCCCAGCACGACAGGGTTT1880.16681159163102696No Hit
GCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCT1880.16681159163102696No Hit
GTGATTTGTCTGCTTAATTGCGATAACGAACGAGACCTCGGCCCTTAAAT1850.16414970453053185No Hit
GTTCTGGGCCGCACGCGCGCTACACTGACAGGGCCAGCGAGTACATCACC1850.16414970453053185No Hit
GCTCTTTCTTGATCTTTTGGATGGTGGTGCATGGCCGTTCTTAGTTGGTG1830.16237511313020178No Hit
GTGCAAAGTACCTTAAAAACATCAACTCAAGAGAAGGGAGTGTGATAAGA1830.16237511313020178No Hit
CGACAGGGTTTAACAAGATTACCCAAACCTCTCGGTTAAGGTGATGTACT1820.16148781743003673No Hit
GAACAGAGAAGCATCAAGGGAGAGTGAGACAAAAAAGAAAGAAAGCGAGA1810.1606005217298717No Hit
CCTCGTTGAAGAGCAATAATTGCAATGCTCTATCCCCAGCACGACAGGGT1770.15705133892921155No Hit
GTCCGCATTTGCGGGCCGCTGGCTTCTTAAGGGGACTATCGGCTCAAGCC1740.15438945182871644No Hit
GGTGATGTACTCGCTGGCCCTGTCAGTGTAGCGCGCGTGCGGCCCAGAAC1700.15084026902805628No Hit
TACTAGGCATTCCTCGTTGAAGAGCAATAATTGCAATGCTCTATCCCCAG1670.14817838192756116No Hit
GGATGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGCTTA1660.14729108622739615No Hit
AATAAGGATTGACAGATTGAGAGCTCTTTCTTGATCTTTTGGATGGTGGT1660.14729108622739615No Hit
ACACTGACAGGGCCAGCGAGTACATCACCTTAACCGAGAGGTTTGGGTAA1640.14551649482706608No Hit
CTTCTTAAGGGGACTATCGGCTCAAGCCGATGGAAGTGCGCGGCAATAAC1620.14374190342673598No Hit
CATCAAGGGAGAGTGAGACAAAAAAGAAAGAAAGCGAGAGAGAGAAAGGG1620.14374190342673598No Hit
GTCCAGCACCCTGCAGGTGATTGTACTAGTGAGAATTGAAATCCACTGGT1600.14196731202640592No Hit
GCACGACAGGGTTTAACAAGATTACCCAAACCTCTCGGTTAAGGTGATGT1600.14196731202640592No Hit
TAACAAGATTACCCAAACCTCTCGGTTAAGGTGATGTACTCGCTGGCCCT1580.14019272062607585No Hit
GGGCATCACAGACCTGTTATTGCCGCGCACTTCCATCGGCTTGAGCCGAT1570.1393054249259108No Hit
GAGCAATAATTGCAATGCTCTATCCCCAGCACGACAGGGTTTAACAAGAT1540.1366435378254157No Hit
GGCCCTGTCAGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCAC1530.13575624212525067No Hit
GCACGAGCTGATGACTCGTGCCTACTAGGCATTCCTCGTTGAAGAGCAAT1530.13575624212525067No Hit
GGCACGAGCTGATGACTCGTGCCTACTAGGCATTCCTCGTTGAAGAGCAA1510.1339816507249206No Hit
GGGACGTAATCGGCACGAGCTGATGACTCGTGCCTACTAGGCATTCCTCG1500.13309435502475556No Hit
GAGCTGATGACTCGTGCCTACTAGGCATTCCTCGTTGAAGAGCAATAATT1500.13309435502475556No Hit
CTTAAATAGCCCGGTCCGCATTTGCGGGCCGCTGGCTTCTTAAGGGGACT1470.13043246792426044No Hit
TCATAGCAGATATTGGTAGATGCTTTTTAGCTAAGCTGAATTGGCTCATT1470.13043246792426044No Hit
GAACATCTAAGGGCATCACAGACCTGTTATTGCCGCGCACTTCCATCGGC1470.13043246792426044No Hit
TAATCGGCACGAGCTGATGACTCGTGCCTACTAGGCATTCCTCGTTGAAG1460.1295451722240954No Hit
TTATATACCTCTGTGTTATGAAAGTAAAATAATTATTCACAAAAATACAA1460.1295451722240954No Hit
AACGAGACCTCGGCCCTTAAATAGCCCGGTCCGCATTTGCGGGCCGCTGG1460.1295451722240954No Hit
GGTATCTATTTTGTATAGACTCTGTGCAAAGTACCTTAAAAACATCAACT1440.12777058082376533No Hit
GCGAAAGCAGGGACGTAATCGGCACGAGCTGATGACTCGTGCCTACTAGG1440.12777058082376533No Hit
CCTGTTATTGCCGCGCACTTCCATCGGCTTGAGCCGATAGTCCCCTTAAG1420.12599598942343526No Hit
ATCTTTGTCTATGAATATGCTATCCCTACAAATTCAGATTGGATGAAGGA1420.12599598942343526No Hit
GGTTAAGGTGATGTACTCGCTGGCCCTGTCAGTGTAGCGCGCGTGCGGCC1410.12510869372327021No Hit
GTTGGTGGAGTGATTTGTCTGCTTAATTGCGATAACGAACGAGACCTCGG1410.12510869372327021No Hit
AGCAGGAACAGAGAAGCATCAAGGGAGAGTGAGACAAAAAAGAAAGAAAG1400.12422139802310518No Hit
GACCTGTTATTGCCGCGCACTTCCATCGGCTTGAGCCGATAGTCCCCTTA1390.12333410232294015No Hit
CTTTCTTGATCTTTTGGATGGTGGTGCATGGCCGTTCTTAGTTGGTGGAG1380.1224468066227751No Hit
CAGCACGACAGGGTTTAACAAGATTACCCAAACCTCTCGGTTAAGGTGAT1370.12155951092261007No Hit
CTTCCATCGGCTTGAGCCGATAGTCCCCTTAAGAAGCCAGCGGCCCGCAA1370.12155951092261007No Hit
GCTCAAGCCGATGGAAGTGCGCGGCAATAACAGGTCTGTGATGCCCTTAG1360.12067221522244503No Hit
ATGAATATGCTATCCCTACAAATTCAGATTGGATGAAGGATCCTTTGATT1350.11978491952227999No Hit
TTGGTGGAGTGATTTGTCTGCTTAATTGCGATAACGAACGAGACCTCGGC1340.11889762382211497No Hit
AGCGAAAGCAGGTGCCCCCTTGTTTTCACACCCCTTTGCCTTATAGATCA1340.11889762382211497No Hit
TCTTAAGGGGACTATCGGCTCAAGCCGATGGAAGTGCGCGGCAATAACAG1330.11801032812194992No Hit
TAAGGGGACTATCGGCTCAAGCCGATGGAAGTGCGCGGCAATAACAGGTC1300.1153484410214548No Hit
TATATACACAGAGAGAGAGAGAGAAATTGATTCTTGGCATCCACAAGGAA1290.11446114532128977No Hit
GGACTTATCTTTGTCTATGAATATGCTATCCCTACAAATTCAGATTGGAT1290.11446114532128977No Hit
CTAGGCATTCCTCGTTGAAGAGCAATAATTGCAATGCTCTATCCCCAGCA1280.11357384962112473No Hit
AATCGGCACGAGCTGATGACTCGTGCCTACTAGGCATTCCTCGTTGAAGA1270.1126865539209597No Hit
GCCCTAATCTCAGCAGGACTTATCTTTGTCTATGAATATGCTATCCCTAC1250.11091196252062963No Hit
GGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGACA1250.11091196252062963No Hit
AGACAAAATAAGGATTGACAGATTGAGAGCTCTTTCTTGATCTTTTGGAT1240.11002466682046459No Hit
CAGCAGGACTTATCTTTGTCTATGAATATGCTATCCCTACAAATTCAGAT1240.11002466682046459No Hit
CATTTGCGGGCCGCTGGCTTCTTAAGGGGACTATCGGCTCAAGCCGATGG1230.10913737112029955No Hit
TTTGCGGGCCGCTGGCTTCTTAAGGGGACTATCGGCTCAAGCCGATGGAA1230.10913737112029955No Hit
CCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGACAGGGCCAGCGAG1220.10825007542013451No Hit
TACCCAAACCTCTCGGTTAAGGTGATGTACTCGCTGGCCCTGTCAGTGTA1220.10825007542013451No Hit
GTGCCTACTAGGCATTCCTCGTTGAAGAGCAATAATTGCAATGCTCTATC1220.10825007542013451No Hit
CCATATTGTACCACTTCAGAATCTTTCGGTTTTTCCCTTGATGCTTCTCT1210.10736277971996946No Hit
GCCTTATAGATCAATGTCTGCTCTTCTTCTTTCCTCCTGTTCCAGAGGAA1210.10736277971996946No Hit
GTCTGCTTAATTGCGATAACGAACGAGACCTCGGCCCTTAAATAGCCCGG1210.10736277971996946No Hit
AGTTGGTGGAGTGATTTGTCTGCTTAATTGCGATAACGAACGAGACCTCG1210.10736277971996946No Hit
GGATGAAGGATCCTTTGATTAGAGATTTAAATGAGGGACAATTTAATGAG1210.10736277971996946No Hit
CGCTGGCCCTGTCAGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCA1190.10558818831963941No Hit
ATATTAATATTTGGGAATCTTCAGGTTGTTTACTCCAGCAGTCCTCCATC1180.10470089261947436No Hit
AAAGCAGGGACGTAATCGGCACGAGCTGATGACTCGTGCCTACTAGGCAT1180.10470089261947436No Hit
CTCCAACACTTTACCTCTCCCTGTTGAGCCAAAAGCAAATCCCAAGACAT1180.10470089261947436No Hit
AATCTCAGCAGGACTTATCTTTGTCTATGAATATGCTATCCCTACAAATT1180.10470089261947436No Hit
TCCATCGGCTTGAGCCGATAGTCCCCTTAAGAAGCCAGCGGCCCGCAAAT1170.10381359691930933No Hit
GTTCTAGAACTTTGGGTGGCTTGTTGCATTTGGGGAATATTAATATTTGG1150.10203900551897925No Hit
GTCTACCACCAAATGCCTCAAAAAACAGCCCTAATCTCAGCAGGACTTAT1150.10203900551897925No Hit
GAAAGCAGGGACGTAATCGGCACGAGCTGATGACTCGTGCCTACTAGGCA1140.10115170981881423No Hit
TCAAGGGAGAGTGAGACAAAAAAGAAAGAAAGCGAGAGAGAGAAAGGGAG1140.10115170981881423No Hit
TAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACAC1140.10115170981881423No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)