FastQCFastQC Report
Wed 12 May 2021
HGGF2BGXJ_n02_ReSeq_Sp19_HK1073_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGF2BGXJ_n02_ReSeq_Sp19_HK1073_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2678113
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC171990.6422059114010499No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGATTCCAACACTGTGTCAAG94400.35248699364067165No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT89260.3332943755547283No Hit
GATACTACTTAGAGCGTTTACAGATGAAGGAGCAATAGTGGGCGAAATCT79210.2957679530326017No Hit
GCGTTTACAGATGAAGGAGCAATAGTGGGCGAAATCTCACCATTACCTTC76980.2874411946023189No Hit
GCATATAGTGGAGCGGATTCTGGAGGAAGAATCTGACGAGGCACTTAAAA59240.2212005243990825No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCATTCTGCTGT58420.21813866703906817No Hit
GTATAACAATAGAGAGAATGGTTCTCTCCGCATTTGATGAAAGGAGGAAC58340.21783994924784728No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAA56090.20943851136975924No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTTCTTA55850.2085423579960965No Hit
AAGCTATTCTCTGTAATTCTTAACCTATGTCGCATTTCTTCAATTAACCA49420.18453291552671602No Hit
GTTCTTATCTCTTGCTCCACTTCAAGCAATAGTTGTAAGGCTTGCATAAA48680.18176977595792262No Hit
GTGCAGACCGTGCCAGAAAGATGAGATCTTCAATTTCAGCATTCCCAGGA48390.1806869239647468No Hit
CTCATATGCAATCCTTGTTCTTCGTCCATTATCGCCTCTCCAGAAGTTCC47080.17579542013350444No Hit
CTTGTGAACAGATTGCTGATGCCCAACATCGGTCCCACAGGCAGATGGCG46630.17411513255788683No Hit
GTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCCGCAAACGATT46390.1732189791842241No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG46370.1731442997364189No Hit
GAGCAAAAGCAGGTAGATGTTGAAAGATGAGTCTTCTAACCGAGGTCGAA46290.17284558194519797No Hit
TTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCTTCAAAC45680.17056785878713857No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT45390.16948500679396275No Hit
GCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATTCTTAACCTATGT43450.16224110035685574No Hit
ATCCAGAACAGTATAACAATAGAGAGAATGGTTCTCTCCGCATTTGATGA43040.1607101716768486No Hit
CTCATAGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA40610.1516366187685135No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGATTCCAACACTGTGTCAAGCT40430.15096450373826648No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG39490.1474545696914208No Hit
GCTTCACGCTACCTAACTGAAATGACTCTTGAGGAAATGTCAAGGGATTG39480.1474172299675182No Hit
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCA39100.14599832045921887No Hit
CTATTGCTCCTTCATCTGTAAACGCTCTAAGTAGTATCAGAGCTTCAAGT38230.14274976447969148No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT37270.1391651509850406No Hit
CTCTTGGTCTGGACATCAGAACTGCCACTCGTGAAGGAAAGCATATAGTG36800.13741018396161775No Hit
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT35770.13356419239964856No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAACATCATGG35700.13330281433233027No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA35120.13113711034597866No Hit
ACAATAGAGAGAATGGTTCTCTCCGCATTTGATGAAAGGAGGAACAGGTA34430.12856066939669833No Hit
CTGTAATTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCT34130.12744047767961994No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTT33880.1265069845820546No Hit
GGTTAATGCTCATTCCCAAGCAGAAAGTGACAGGGCCCCTTTGCATTAGA33320.12441596004350824No Hit
GGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATG32790.12243695467666973No Hit
GGTGCGCTTGCCAGTTGCATGGGTCTCATATACAACCGGATGGGAACAGT32430.12109272461617565No Hit
CTCTTGCTCCACTTCAAGCAATAGTTGTAAGGCTTGCATAAAGGTTATTT32090.1198231740034868No Hit
CTATAGGACTTGCAAGCTAGTTGGAATCAACATGAGCAAAAAGAAGTCTT31520.11769480974103781No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC31350.1170600344346934No Hit
GTATGACAAAGAGGAGATAAGGAGAATTTGGCGTCAAGCGAACAATGGAG30800.11500634962004964No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG30430.11362477983565294No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA30360.11336340176833465No Hit
ACTCAAAAGGCAATTGATAAAATAACATCCAAGGTGAATAATATAGTCGA30150.11257926756637975No Hit
GATGTGCACTGAACTCAAACTCAGCGACCAAGAAGGAAGGTTGATCCAGA28790.10750106511562432No Hit
GTTACTCAACTGGTGCGCTTGCCAGTTGCATGGGTCTCATATACAACCGG28160.10514866250975967No Hit
GTCCTGGAAGGGAAGGTAATGGTGAGATTTCGCCCACTATTGCTCCTTCA27880.10410315024048648No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTTCTTATCTCTTGCTC27660.10328167631462898No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA27390.10227350376925844No Hit
CAACATGGACAGGGCAGTTAAACTATACAAGAAGCTGAAGAGGGAAATGA27380.1022361640453558No Hit
ACATAATGGATTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTT27360.10216148459755059No Hit
GAACTGGGTGATGCCCCATTCCTTGACCGGCTTCGCCGAGATCAGAAGTC27300.10193744625413492No Hit
GTCATATTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA27220.101638728462914No Hit
GCGAAAGCAGGCACTGAAGCGATAGTCATTTTAAGTGCCTCGTCAGATTC27130.10130267094779048No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)