FastQCFastQC Report
Wed 12 May 2021
HGGF2BGXJ_n02_ReSeq_F17_HK1073_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGF2BGXJ_n02_ReSeq_F17_HK1073_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences708850
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC55780.7869083727163715No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGATTCCAACACTGTGTCAAG38840.5479297453622064No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAA28520.40234182125978696No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT27630.38978627354165196No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT22030.3107850744163081No Hit
GAGCAAAAGCAGGTAGATGTTGAAAGATGAGTCTTCTAACCGAGGTCGAA19940.28130069831417087No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG19740.27847922691683713No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTTTACTTT19130.2698737391549693No Hit
GCATATAGTGGAGCGGATTCTGGAGGAAGAATCTGACGAGGCACTTAAAA18930.2670522677576356No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT18770.26479509063976864No Hit
TTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCTTCAAAC18550.26169147210270155No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG17940.25308598434083374No Hit
CTATTGCTCCTTCATCTGTAAACGCTCTAAGTAGTATCAGAGCTTCAAGT17590.24814840939549973No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGATTCCAACACTGTGTCAAGCT16470.23234816957043097No Hit
AAGCTATTCTCTGTAATTCTTAACCTATGTCGCATTTCTTCAATTAACCA15620.2203569161317627No Hit
GTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCCGCAAACGATT15220.2147139733370953No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCATTCTGCTGT15160.2138675319178952No Hit
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT15050.21231572264936166No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA15000.2116103548000282No Hit
CTCTTGGTCTGGACATCAGAACTGCCACTCGTGAAGGAAAGCATATAGTG14780.20850673626296112No Hit
GCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATTCTTAACCTATGT14200.20032446921069338No Hit
GTTCTTATCTCTTGCTCCACTTCAAGCAATAGTTGTAAGGCTTGCATAAA13990.197361924243493No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTTCTTA13390.18889751005149183No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCT13340.18819214220215844No Hit
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCA13320.18790999506242506No Hit
ACCTAACTGAAATGACTCTTGAGGAAATGTCAAGGGATTGGTTAATGCTC13190.18607603865415814No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG13160.1856528179445581No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA13100.18480637652535797No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC13020.18367778796642448No Hit
GTAATGGGTAAAACCATCATATTGAAAGCAAACTTTAGTGTGATTTTTAA12930.1824081258376243No Hit
GTCCTGGAAGGGAAGGTAATGGTGAGATTTCGCCCACTATTGCTCCTTCA12800.18057416942935742No Hit
AAGTAGTATCAGAGCTTCAAGTCGATTAAAAATCACACTAAAGTTTGCTT12630.17817591874162375No Hit
GGTTAATGCTCATTCCCAAGCAGAAAGTGACAGGGCCCCTTTGCATTAGA12430.17535444734429004No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA12070.17027579882908936No Hit
AGAATGGACCAGGCAGTAATGGGTAAAACCATCATATTGAAAGCAAACTT11710.1651971503138887No Hit
CCCTAAGAGGAAGAGGCAGCACTCTTGGTCTGGACATCAGAACTGCCACT11660.16449178246455526No Hit
GGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATG11650.16435070889468859No Hit
GATACTACTTAGAGCGTTTACAGATGAAGGAGCAATAGTGGGCGAAATCT11320.15969528108908795No Hit
CTGTAATTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCT11220.1582845453904211No Hit
CTCATAGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA11210.1581434718205544No Hit
CTCTTGCTCCACTTCAAGCAATAGTTGTAAGGCTTGCATAAAGGTTATTT11100.1565916625520209No Hit
GCGTTTACAGATGAAGGAGCAATAGTGGGCGAAATCTCACCATTACCTTC11100.1565916625520209No Hit
CTTGTGAACAGATTGCTGATGCCCAACATCGGTCCCACAGGCAGATGGCG10880.15348804401495378No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTT10850.15306482330535376No Hit
GCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT10770.15193623474642026No Hit
ACTTAGAGCGTTTACAGATGAAGGAGCAATAGTGGGCGAAATCTCACCAT10550.14883261620935317No Hit
GCAAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGA10110.142625379135219No Hit
GAGTGGAGATCTCCCATTCTCATCACTGCTTCTCCAAGTGAATCTCTGTA10070.14206108485575228No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC9840.13881639274881852No Hit
ATATAGTGGAGCGGATTCTGGAGGAAGAATCTGACGAGGCACTTAAAATG9780.1379699513296184No Hit
GAACTGGGTGATGCCCCATTCCTTGACCGGCTTCGCCGAGATCAGAAGTC9710.1369824363405516No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT9570.135007406362418No Hit
ACCCATTACTGCCTGGTCCATTCTAATGCAAAGGGGCCCTGTCACTTTCT9450.1333145235240178No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT9440.1331734499541511No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT9240.13035197855681738No Hit
CTCCCATTCTCATCACTGCTTCTCCAAGTGAATCTCTGTAGAGTTTCAGA8970.12654299217041687No Hit
TTCCTTGACCGGCTTCGCCGAGATCAGAAGTCCCTAAGAGGAAGAGGCAG8950.1262608450306835No Hit
GTTACTCAACTGGTGCGCTTGCCAGTTGCATGGGTCTCATATACAACCGG8910.12569655075121675No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGATTCCAACACTGTGTCAAGC8880.1252733300416167No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT8810.1242858150525499No Hit
ACATAATGGATTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTT8700.12273400578401636No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATGCATTTGAAGAAAAGACG8630.12174649079494956No Hit
GTACTAGCCAGCACTACGGCTAAGGCCATGGAGCAGATGGCTGGATCAAG8540.1204768286661494No Hit
GGGTAAAACCATCATATTGAAAGCAAACTTTAGTGTGATTTTTAATCGAC8490.11977146081681596No Hit
ATCAAGTGCAATATCCCAATGATACTTGCTGCAACAACGAGAGGATCACT8400.11850179868801579No Hit
GTATCAGAGCTTCAAGTCGATTAAAAATCACACTAAAGTTTGCTTTCAAT8350.11779643083868238No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA8260.11652676870988221No Hit
ATCCCAATGATACTTGCTGCAACAACGAGAGGATCACTTGAATCTCTGCA8250.11638569514001552No Hit
CTTAAAATGACTATCGCTTCAGTGCCTGCTTCACGCTACCTAACTGAAAT8050.11356422374268181No Hit
GAACTAGGGTGAGTCCCAATTGTCCTCATAGCCTGCACCATTTGCCTAGC8040.11342315017281512No Hit
TCTCTGTAGAGTTTCAGAGACTCGAACTGTGTTATCATTCCATTCAAGTC8040.11342315017281512No Hit
CAACATGGACAGGGCAGTTAAACTATACAAGAAGCTGAAGAGGGAAATGA7870.11102489948508147No Hit
GGCTAGTACCATTCTGTTCTCATGCCTGATTAGTGGGTTGGTGGTAGTCG7870.11102489948508147No Hit
CTGTAGAGTTTCAGAGACTCGAACTGTGTTATCATTCCATTCAAGTCCTC7810.11017845806588135No Hit
GTTTTGGAGGGAGTGGAGATCTCCCATTCTCATCACTGCTTCTCCAAGTG7810.11017845806588135No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCCTGTCACCTCTGACTAAGGG7650.1079212809480144No Hit
TTCTAATGCAAAGGGGCCCTGTCACTTTCTGCTTGGGAATGAGCATTAAC7600.10721591309868095No Hit
CTTCAGTGCCTGCTTCACGCTACCTAACTGAAATGACTCTTGAGGAAATG7540.10636947167948084No Hit
CTCATATACAACCGGATGGGAACAGTGACCACAGAAGTGGCTCTTGGCCT7390.10425336813148056No Hit
CTTCAAACTTCTGGCTCAATTGTTCTCTCCACTTTCCGTTTCTGTTTTGG7340.10354800028214714No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAG7310.10312477957254709No Hit
CTATCATCCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTTGAG7250.10227833815334697No Hit
CTTTTAGACCTGCACTGGAACTAGGGTGAGTCCCAATTGTCCTCATAGCC7250.10227833815334697No Hit
GTCATATTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA7220.10185511744374692No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTTCTTATCTCTTGCTC7170.10114974959441347No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)