FastQCFastQC Report
Wed 12 May 2021
HGGF2BGXJ_n02_ReSeq_2231_d04_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGF2BGXJ_n02_ReSeq_2231_d04_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3966320
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAATTGATATGCAAAAAACCCCGGCCTTATTGACCGGGGTTTTTCT335180.8450654510982472No Hit
GAGCAAAAGCAGGACGGATAAGCAAAAGTAGGCTGGATAAGACGCGCCAG278100.7011537142741887No Hit
GGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACG258480.6516872062768511No Hit
GGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGAT231430.5834879686964239No Hit
GGATAAGACGCGCCAGCGTCGCATCCGGTATTAATTGATATGCAAAAAAC202030.5093638435627987No Hit
GAGCAAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATC196790.49615260493354035No Hit
GTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGC182080.4590653300792675No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGA180990.45631719074608207No Hit
GTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCTTCGGCGTTGT177190.4467365215111237No Hit
CATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTA154590.3897567518505819No Hit
CGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTG149700.3774279432824381No Hit
GATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATA147770.3725619718025777No Hit
AATCTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCT138590.3494170919139151No Hit
TCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGG137070.34558482421993186No Hit
ATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGT131120.3305835131809839No Hit
GCCTTATTGACCGGGGTTTTTCTAATTTGATGCCTGGCAGTTCCCTACTC128940.3250872345146131No Hit
GTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGA128650.32435607817826095No Hit
TCATTAACCCGCTTGTCGCCGGTTAATCTAATCTGTATCAGGCTGAAAAT126890.3199187155852276No Hit
GTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTT126390.3186581012122068No Hit
AATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCTTCGGC123320.3109179289618588No Hit
CATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAA118720.29932027673006717No Hit
GACTTAGAGTCTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCG117030.29505940014925675No Hit
CTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGATGTTTTGGCGGA116260.2931180540148047No Hit
GGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTCATTAACCC114120.28772262449827546No Hit
GTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGATGTTTTGGC112040.2824784687065088No Hit
GGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCG109600.2763266705661671No Hit
GCAAAAGCAGGACGGATAAGCAAAAGTAGGCTGGATAAGACGCGCCAGCG108850.27443574900663587No Hit
CTCTAAGTCTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATG103310.26046814175356503No Hit
ACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTG102880.2593840133927671No Hit
TCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGT100510.2534087012646483No Hit
TCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAATG99560.25101353395590875No Hit
GTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGAT99460.25076141108130456No Hit
GGGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTCAT99360.25050928820670043No Hit
CGCTAAGGCCGCCAGGCAAATTCTGTTTCATTAACCCGCTTGTCGCCGGT91790.23142358659916495No Hit
ATCTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCTT88840.22398596179834204No Hit
CCCTGAGACTTAGAGTCTCCTGAAGGAACGTTGAAGACGACGACGTTGAT86290.2175568284959358No Hit
TCCTGGGGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGT86140.21717864418402955No Hit
GTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTA84080.21198491296718366No Hit
GAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAG82730.20858125416002743No Hit
TAGTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTGTCATG81450.2053540813650941No Hit
CTATTGCACCGTTCGTGACAGTAAAGATGTGGTAAACACCACTGAGCAGG80660.2033623106557212No Hit
TTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATC79520.20048810988523366No Hit
GATAAGTGCTGAAAGCATCTAAGCACGAAACTTGCCCCGAGATGAGTTCT78430.19773997055204826No Hit
AGCGAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCG78420.19771475826458784No Hit
AAGCAGGACGGATAAGCAAAAGTAGGCTGGATAAGACGCGCCAGCGTCGC77180.19458843461949615No Hit
GTGCTGAAAGCATCTAAGCACGAAACTTGCCCCGAGATGAGTTCTCCCTG77120.19443716089473367No Hit
GTTTGGCACCTCGATGTCGGCTCATCACATCCTGGGGCTGAAGTAGGTCC74820.18863833477883782No Hit
GTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGG74680.188285362754392No Hit
AGCAAAAGTAGGCTGGATAAGACGCGCCAGCGTCGCATCCGGTATTAATT74490.18780632929264407No Hit
CCTAGTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTGTCA73080.1842513967607253No Hit
AGCGAAAGCAGGACGGATAAGCAAAAGTAGGCTGGATAAGACGCGCCAGC72190.18200750317674824No Hit
GAGTAATACAGTGCTATCAAGCTGTTCAAGTGCCATACTGACATACCCTG72140.18188144173944615No Hit
GTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAATGCGGAAG71980.18147804514007948No Hit
GCCCGGTAGCTAAATGCGGAAGAGATAAGTGCTGAAAGCATCTAAGCACG71200.17951148671816697No Hit
GCAAAAAACCCCGGCCTTATTGACCGGGGTTTTTCTAATTTGATGCCTGG69130.1742925432138607No Hit
GGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGT69130.1742925432138607No Hit
GCAAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGC68640.17305714112830028No Hit
CATCACATCCTGGGGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCAT68340.17230077250448778No Hit
CAATGGCACTGCCCGGTAGCTAAATGCGGAAGAGATAAGTGCTGAAAGCA67920.17124185643115028No Hit
GTCTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATGCTTTCA67540.17028378950765446No Hit
GAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAG67230.16950220859638154No Hit
GTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCC67200.16942657173400028No Hit
AAAGTAGGCTGGATAAGACGCGCCAGCGTCGCATCCGGTATTAATTGATA67030.1689979628471732No Hit
TATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGA67010.16894753827225237No Hit
CCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGG66750.16829201879828154No Hit
GAGTGGACGCATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCG65810.16592206377700236No Hit
CTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCGGGTGTGTAAG65500.16514048286572944No Hit
GGTTAATCTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAAC65140.16423284051715445No Hit
GGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTG65080.16408156679239194No Hit
GTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC64940.1637285947679461No Hit
CTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGAG63890.16108130458460235No Hit
GTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTGTCATGCC63130.1591651707376107No Hit
GTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCAT62020.15636660682950443No Hit
ATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAA60670.15296294802234817No Hit
GCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCAC60340.15213094253615442No Hit
GTCATGCCAATGGCACTGCCCGGTAGCTAAATGCGGAAGAGATAAGTGCT60230.15185360737408984No Hit
GCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCA59240.1493575909155086No Hit
GGAGTGGACGCATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCC58320.14703806046915024No Hit
CTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACG58120.14653381471994192No Hit
TCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCA57950.14610520583311484No Hit
AAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGAC57480.14492022832247523No Hit
GGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTT57150.14408822283628148No Hit
ACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATG57150.14408822283628148No Hit
CTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTAAGGCCGCCA55810.14070977631658566No Hit
GCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCT55490.13990298311785232No Hit
GTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGGGCTGAAGTAGGT55460.13982734625547108No Hit
GATTAGATTAACCGGCGACAAGCGGGTTAATGAAACAGAATTTGCCTGGC54790.13813812299562314No Hit
GTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAAC53960.13604550313640856No Hit
GAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCATCACTGGT53590.13511264850037313No Hit
CTATTGCACCGTTCGTGACACAGACTCGGGATCAATTTAGCTTTGTTGAG52620.13266705661671271No Hit
GGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTCATT51450.12971721898384397No Hit
GAGCAAAAGCAGGTCGCGGCGTATATTTTGGCGGCTCAGATTTTGCCTGC51450.12971721898384397No Hit
CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGG51410.12961636983400232No Hit
GTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCC50420.12712035337542105No Hit
CTTCAGGAGACTCTAAGTCTCAGGGAGAACTCATCTCGGGGCAAGTTTCG49410.12457391234191896No Hit
CCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGG49380.12449827547953769No Hit
GGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCG49080.12374190685572521No Hit
GCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGAGACTCTAAGTCTC48140.12137195183444603No Hit
TTCCATAACTGTCTCAAAAGTGACCATAAGCTCTCCTGAATCAGGAGAGC47570.11993485144920228No Hit
GAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAG46850.11811956675205226No Hit
GCCTGATACAGATTAGATTAACCGGCGACAAGCGGGTTAATGAAACAGAA46250.11660682950442727No Hit
GTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTC45710.11524536598156478No Hit
GGGTTTTTCTAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGA45520.11476633251981687No Hit
TAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAG45100.11370741644647936No Hit
GGCAAATTCTGTTTCATTAACCCGCTTGTCGCCGGTTAATCTAATCTGTA44950.1133292321345731No Hit
TTGATAGGCCGGGTGTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACT44730.11277456181044394No Hit
AGCAAAAGCAGGACGGATAAGCAAAAGTAGGCTGGATAAGACGCGCCAGC44520.1122451037737752No Hit
ATCACATCCTGGGGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATT44440.11204340547409186No Hit
GAAGAGATAAGTGCTGAAAGCATCTAAGCACGAAACTTGCCCCGAGATGA44370.11186691946186893No Hit
CCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTT44160.11133746142520018No Hit
GGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCA44000.11093406482583351No Hit
GGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACC42710.10768167974343976No Hit
GCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACC41450.10450493152342727No Hit
CTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAATGCGG41390.10435365779866476No Hit
CGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGG40430.10193327820246474No Hit
CAAAAAACCCCGGCCTTATTGACCGGGGTTTTTCTAATTTGATGCCTGGC40280.1015550938905585No Hit
TCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTT40070.10102563585388975No Hit
TCCTTCAGGAGACTCTAAGTCTCAGGGAGAACTCATCTCGGGGCAAGTTT39890.10057181467960224No Hit
ACCTTACAACGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCT39810.10037011637991891No Hit
GTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATC39710.10011799350531476No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)