FastQCFastQC Report
Wed 12 May 2021
HGGF2BGXJ_n02_ReSeq_1984_d10_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGF2BGXJ_n02_ReSeq_1984_d10_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences815373
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAAGGCTATGTATTGAGCACGCAGGAGTGGGTGCCTTTTAGGGGGAG14470.17746479218713399No Hit
GAGTTAAAGGTTTGTAAAGGAAAATGGTTAAAAAAAGAGAGAGAAAGAAA13380.16409667722625104No Hit
GTCCATGTGTGGCCACGTGCTCAATACAGCCACCTCCACATGGAAAGCCT10970.13453965240448235No Hit
GTATTGAGCACGCAGGAGTGGGTGCCTTTTAGGGGGAGGCCCCCAAGCGT10360.12705841375664878No Hit
GAGCACCCCTGCCTATGTTGGGGCTTCCTGTCCAAGGCTATGTATTGAGC9860.12092625093055571No Hit
TCCCTATCCAGAGCAGAGTTAGGCTTTGAATGGCAGGTGGGCCCCAGCCA9690.11884131556968405No Hit
GTGTTACTCTGTCCAGAAGCTTACCAGGGAAGGGAAGAAAAAGCATGGTT9400.11528466113055007No Hit
GTATATGGAGAAAGCAGTTGTTTTGAGTCCTGGAGTTTTAGAGAAAATGG9280.11381294205228773No Hit
GTTCTAGGGCATGATTGCATGTTGGTTCATGAAAAGCGCACAGAGCACCC8850.10853928202184766No Hit
GCTATGTATTGAGCACGCAGGAGTGGGTGCCTTTTAGGGGGAGGCCCCCA8750.10731284945662906No Hit
GTCCAGAAGCTTACCAGGGAAGGGAAGAAAAAGCATGGTTTCAGTGCCAT8550.10485998432619181No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)