FastQCFastQC Report
Wed 12 May 2021
HGGF2BGXJ_n02_ReSeq_1971_d04_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGF2BGXJ_n02_ReSeq_1971_d04_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96489
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAACTCAGCACCTTATTCAAATCCTGTTGGAAGCCAGTTCTCCTT5940.6156142150918757No Hit
CCATAGAAGAGGGCTTTGCTTCTCAAGTCGCAGCTGGGAGACCCTGGATC4580.47466550591259105No Hit
GTCCCAAGACTGTCTCCTGATAAGAGCTTTTTGCTGTGGTACCCCGAGGG4490.4653380178051384No Hit
CCCCCGACTCGTGCAAGCCTGGGGAGGGGCCGCGAAGGTGTTTTCCCCCA4250.4404647161852646No Hit
GCCCTGAGCAGAGGCAACAGAGGCAGAAAAATGCCAGGATGCCCTGGCAG4020.4166278021328856No Hit
GTCATGTATCATGCTAATTGAAATGATGGGCTATTCTTCTAGGTGTACAA3590.37206313673061175No Hit
CACATGGGGAGGTGGCCACCAAGGCTGATGGTGGCAGGACCAGTGCTGAG3540.3668811988931381No Hit
GCCTGGCCCTGAGCAGAGGCAACAGAGGCAGAAAAATGCCAGGATGCCCT3520.3648084237581486No Hit
GTGCTGAGCCCGATGAGCTTCTGGAGGCAGGAGGAGCAGGAGGCGGCCCT3490.36169926105566436No Hit
ATTCAAATCCTGTTGGAAGCCAGTTCTCCTTCGAGTGCCAGCCTGGCCCT3390.35133538538071696No Hit
AGCCTGGGGAGGGGCCGCGAAGGTGTTTTCCCCCATGGACCAGAACTCAG3070.3181709832208853No Hit
CCCTGGATCCTTGCCACTGTCAAAAGAAAAAGCACCCACAGAGGCAGGGT3070.3181709832208853No Hit
TCTTTATACTTTGACTTATTCAGTTAACAGAGAGGACTAAAGGTGACAGT2950.30573433241094844No Hit
GTGCAAGCCTGGGGAGGGGCCGCGAAGGTGTTTTCCCCCATGGACCAGAA2850.295370456736001No Hit
GAAGAGGGCTTTGCTTCTCAAGTCGCAGCTGGGAGACCCTGGATCCTTGC2830.29329768160101155No Hit
ACTCAGCACCTTATTCAAATCCTGTTGGAAGCCAGTTCTCCTTCGAGTGC2760.28604296862854833No Hit
GCAATAGGCTTTGCTCTGAAAGTGCACTTGGGAGAGATTGGGTGGCACGT2730.2829338059260641No Hit
GACCAGTGCTGAGCCCGATGAGCTTCTGGAGGCAGGAGGAGCAGGAGGCG2630.2725699302511167No Hit
GCCTGGTTCATGCCATAGAAGAGGGCTTTGCTTCTCAAGTCGCAGCTGGG2520.26116966700867456No Hit
GAACTCAGCACCTTATTCAAATCCTGTTGGAAGCCAGTTCTCCTTCGAGT2510.2601332794411798No Hit
GTACTAAACAAATTTAACCTAAGTAGTGAAATTTTGCAGTTTATGAGAAG2460.25495134160370614No Hit
CTCTGGGGTGGCGAGAGGGAAAGGGAGCTTCTGGCCAGGGATTGGTCTTG2430.2518421789012219No Hit
GACTTATTCAGTTAACAGAGAGGACTAAAGGTGACAGTGATGGGGACTTT2220.23007803998383236No Hit
GCCAAGACCAATCCCTGGCCAGAAGCTCCCTTTCCCTCTCGCCACCCCAG2190.22696887728134812No Hit
GTGGTACCCCGAGGGGCTGCTGAGGGGGGGACCTTTGTGGGCTTTGCCAC2140.22178693944387443No Hit
CCGTACAGCCGTCCTGAGGGGCATGCACCCTCGAAGGGGCCAACACTTGG2080.21556861403890598No Hit
GAGCAGAGGCAACAGAGGCAGAAAAATGCCAGGATGCCCTGGCAGCAGAT2070.21453222647141124No Hit
GTCCTTGGCTGAGGCTTGCCCCTTACCGACTGGCTTTGGCACGCTGGGCG2050.21245945133642175No Hit
ACCCCAGAGTGGCCAGGCAGTGGCAAAGCCCACAAAGGTCCCCCCCTCAG2010.2083139010664428No Hit
GTCCCCATGCTGTGTCCCAAGACTGTCTCCTGATAAGAGCTTTTTGCTGT2000.20727751349894807No Hit
CCCCAGTGGTGCTCTCTCATCCTCTGGTGCTATCGACCAAAACCCAAGCA1960.2031319632289691No Hit
CATGGGGAGGTGGCCACCAAGGCTGATGGTGGCAGGACCAGTGCTGAGCC1920.19898641295899014No Hit
GTAATATGGATGGCTGCTGTGCAGCCTTAGATTTCCCTCTTGACTTCTGT1880.19484086268901118No Hit
TATTAGAACCCTCTGGAAGGAAATATTTAAGTAACACTTTAGATGAAAAC1780.18447698701406376No Hit
GTCTTGGGACACAGCATGGGGACCCTGCCTCTGTGGGTGCTTTTTCTTTT1770.18344059944656904No Hit
GTCAAAAGAAAAAGCACCCACAGAGGCAGGGTCCCCATGCTGTGTCCCAA1730.1792950491765901No Hit
GTTTATGAGAAGTGTGATTTTGATTACCTTGTTTCTGTAGATTCTGTTGT1700.17618588647410585No Hit
GCCCACTGCCGTGCTACAAGGGCAGGAGCGAGCATCCCACCGACCCCAGT1690.1751494989066111No Hit
TTTTTAGCCTTTTAATGCTACAGGATTATTTTTCTCAACTAAGTTCATCC1670.17307672377162164No Hit
CCCCACACCGCAGCCCACTGCCGTGCTACAAGGGCAGGAGCGAGCATCCC1660.1720403362041269No Hit
GTGTACTATCAGAACTTTCATCCAGTTATTGTTTAACATTGCTTAAGTCT1640.1699675610691374No Hit
TATAGAAGCAGATCCAATATTTTCCAGGTAATAGACATCCTGGAACTCAA1640.1699675610691374No Hit
GTTCATGCCATAGAAGAGGGCTTTGCTTCTCAAGTCGCAGCTGGGAGACC1620.16789478593414794No Hit
CCCCAGAGTGGCCAGGCAGTGGCAAAGCCCACAAAGGTCCCCCCCTCAGC1580.16374923566416896No Hit
GGGCAGGAGCGAGCATCCCACCGACCCCAGTGGTGCTCTCTCATCCTCTG1560.1616764605291795No Hit
ACCTGCAGGGGGGCTGCAATAGGCTTTGCTCTGAAAGTGCACTTGGGAGA1560.1616764605291795No Hit
GATACACCTCGCTCCTGCGGTTCCCACTCCCAACAGGATGGAGCTGCCAA1550.16064007296168475No Hit
GGGTACCACAGCAAAAAGCTCTTATCAGGAGACAGTCTTGGGACACAGCA1540.15960368539419No Hit
GCTGAGGGGGGGACCTTTGTGGGCTTTGCCACTGCCTGGCCACTCTGGGG1540.15960368539419No Hit
GGGGAAGGCAGCGGGGATTCACATGGGGAGGTGGCCACCAAGGCTGATGG1530.15856729782669526No Hit
GTGCTGGGGGAGGAATGCCGGTCTCAAAAGGCACTGATCCCGTGCTACCT1520.15753091025920052No Hit
GTTTTCCCCCATGGACCAGAACTCAGCACCTTATTCAAATCCTGTTGGAA1510.1564945226917058No Hit
TTTTAATGCTACAGGATTATTTTTCTCAACTAAGTTCATCCAGACTAGAC1510.1564945226917058No Hit
TCTGGAGGCAGGAGGAGCAGGAGGCGGCCCTGCCTGGTTCATGCCATAGA1490.1544217475567163No Hit
CAGATACACCTCGCTCCTGCGGTTCCCACTCCCAACAGGATGGAGCTGCC1470.15234897242172682No Hit
GTTAACAGAGAGGACTAAAGGTGACAGTGATGGGGACTTTTGAGAAGCAG1440.1492398097192426No Hit
CAGTTAACAGAGAGGACTAAAGGTGACAGTGATGGGGACTTTTGAGAAGC1440.1492398097192426No Hit
CCTGGGGAGGGGCCGCGAAGGTGTTTTCCCCCATGGACCAGAACTCAGCA1440.1492398097192426No Hit
ATAGAAGAGGGCTTTGCTTCTCAAGTCGCAGCTGGGAGACCCTGGATCCT1420.14716703458425312No Hit
CCTTATTCAAATCCTGTTGGAAGCCAGTTCTCCTTCGAGTGCCAGCCTGG1390.1440578718817689No Hit
GTTGGAAGCCAGTTCTCCTTCGAGTGCCAGCCTGGCCCTGAGCAGAGGCA1380.14302148431427417No Hit
CTTTAGATGAAAACACAAAGTCATGTATCATGCTAATTGAAATGATGGGC1370.14198509674677942No Hit
GTCTGACAGTGATAAGACAAGGGACAGTGGCTTTAAACTAAATGAGGGGA1370.14198509674677942No Hit
CTACAGGATTATTTTTCTCAACTAAGTTCATCCAGACTAGACACACAAAT1370.14198509674677942No Hit
ACCTTATTCAAATCCTGTTGGAAGCCAGTTCTCCTTCGAGTGCCAGCCTG1370.14198509674677942No Hit
TTTGTGGGCTTTGCCACTGCCTGGCCACTCTGGGGTGGCGAGAGGGAAAG1360.14094870917928468No Hit
GGATTATTTTTCTCAACTAAGTTCATCCAGACTAGACACACAAATATTGA1360.14094870917928468No Hit
ATATATAGAAGCAGATCCAATATTTTCCAGGTAATAGACATCCTGGAACT1340.1388759340442952No Hit
GCCATGAAATGCTCCTTTTCAGGCAAGTAATATGGATGGCTGCTGTGCAG1330.13783954647680047No Hit
CTCCCAACAGGATGGAGCTGCCAAGACCAATCCCTGGCCAGAAGCTCCCT1330.13783954647680047No Hit
GAGGCAACAGAGGCAGAAAAATGCCAGGATGCCCTGGCAGCAGATACACC1310.13576677134181098No Hit
ATACATGACTTTGTGTTTTCATCTAAAGTGTTACTTAAATATTTCCTTCC1300.13473038377431623No Hit
ATATTGAAGTGTTTGTAACAACAGAATCTACAGAAACAAGGTAATCAAAA1290.1336939962068215No Hit
CTCTGGGCACTTTGCCCTACCAGTTCCCCTTCCAGCTGCTGCACGAGGCG1290.1336939962068215No Hit
AAGCAGGGCCATGAAATGCTCCTTTTCAGGCAAGTAATATGGATGGCTGC1260.13058483350433728No Hit
CTCCTGATAAGAGCTTTTTGCTGTGGTACCCCGAGGGGCTGCTGAGGGGG1260.13058483350433728No Hit
GTCATTTGCTATGTGTACTATCAGAACTTTCATCCAGTTATTGTTTAACA1260.13058483350433728No Hit
GTAATAGACATCCTGGAACTCAAGCTCCTGCTCTCTGAATGTTATTAGTT1250.12954844593684253No Hit
GAGCAGGAGGCGGCCCTGCCTGGTTCATGCCATAGAAGAGGGCTTTGCTT1250.12954844593684253No Hit
AGCGAAAGCAGGGCCATGAAATGCTCCTTTTCAGGCAAGTAATATGGATG1240.1285120583693478No Hit
GCCTTAGATTTCCCTCTTGACTTCTGTCTTGGCCAGCAGAATTCTGAGTG1240.1285120583693478No Hit
GTTCTCCTTCGAGTGCCAGCCTGGCCCTGAGCAGAGGCAACAGAGGCAGA1230.12747567080185307No Hit
GGATGGAGCTGCCAAGACCAATCCCTGGCCAGAAGCTCCCTTTCCCTCTC1220.12643928323435832No Hit
GATCCCGTGCTACCTGCAGGGGGGCTGCAATAGGCTTTGCTCTGAAAGTG1210.12540289566686358No Hit
CTGCAGGGGGGCTGCAATAGGCTTTGCTCTGAAAGTGCACTTGGGAGAGA1210.12540289566686358No Hit
CCTTAAGCCTGACCACCACCCATCTGACGCCCTTGGTTTTGCAATACAAG1210.12540289566686358No Hit
CTCTCTGGGCACTTTGCCCTACCAGTTCCCCTTCCAGCTGCTGCACGAGG1200.12436650809936885No Hit
CTTCTGGAGGCAGGAGGAGCAGGAGGCGGCCCTGCCTGGTTCATGCCATA1200.12436650809936885No Hit
CTCTGGAAGGAAATATTTAAGTAACACTTTAGATGAAAACACAAAGTCAT1190.1233301205318741No Hit
GTATTGAGTTTAGTGAGAAAATTCACCCTTGCGATTCTGAAAGTGGTTTT1190.1233301205318741No Hit
AGTTTACACACGCCCAGCGTGCCAAAGCCAGTCGGTAAGGGGCAAGCCTC1170.12125734539688462No Hit
TATCAAAAGTGATTATTAGAACCCTCTGGAAGGAAATATTTAAGTAACAC1170.12125734539688462No Hit
CAACAGGATGGAGCTGCCAAGACCAATCCCTGGCCAGAAGCTCCCTTTCC1160.12022095782938988No Hit
AATTTAACCTAAGTAGTGAAATTTTGCAGTTTATGAGAAGTGTGATTTTG1150.11918457026189515No Hit
CTCTCATCCTCTGGTGCTATCGACCAAAACCCAAGCACTTTCCAAGAAAA1150.11918457026189515No Hit
GACTAAAGGTGACAGTGATGGGGACTTTTGAGAAGCAGCTAAAAATCCCA1130.11711179512690566No Hit
CTACAAGGGCAGGAGCGAGCATCCCACCGACCCCAGTGGTGCTCTCTCAT1130.11711179512690566No Hit
GATTAGGATAGATCATAGAATCACAGAATGGCTTAAGTTGGAGGGGACCT1120.11607540755941093No Hit
ATATAGAAGCAGATCCAATATTTTCCAGGTAATAGACATCCTGGAACTCA1100.11400263242442144No Hit
CTCATAAACTGCAAAATTTCACTACTTAGGTTAAATTTGTTTAGTACCAT1090.1129662448569267No Hit
GATTCACATGGGGAGGTGGCCACCAAGGCTGATGGTGGCAGGACCAGTGC1080.11192985728943196No Hit
GGCTGATGGTGGCAGGACCAGTGCTGAGCCCGATGAGCTTCTGGAGGCAG1070.11089346972193721No Hit
TCCCTATACATGATAGCCAGCTCAGCATTTGTACTTACAATAAAACAAGA1060.10985708215444248No Hit
TTAGAACCCTCTGGAAGGAAATATTTAAGTAACACTTTAGATGAAAACAC1060.10985708215444248No Hit
GACCAATCCCTGGCCAGAAGCTCCCTTTCCCTCTCGCCACCCCAGAGTGG1060.10985708215444248No Hit
ACCCACCCCCGACTCGTGCAAGCCTGGGGAGGGGCCGCGAAGGTGTTTTC1040.10778430701945299No Hit
GTGTATGGCTTTGTGAGGGAGTGCCTGGAGCGGGTGATTCCTGCCGAGCT1040.10778430701945299No Hit
GCCTAAAACAGCAAAAGTGGAGAAAGAAGAGGTTGCCCAAACGCTACTGG1040.10778430701945299No Hit
CCTAGAAGAATAGCCCATCATTTCAATTAGCATGATACATGACTTTGTGT1030.10674791945195826No Hit
GCCCACCCCACACCGCAGCCCACTGCCGTGCTACAAGGGCAGGAGCGAGC1030.10674791945195826No Hit
AACCTAAGTAGTGAAATTTTGCAGTTTATGAGAAGTGTGATTTTGATTAC1030.10674791945195826No Hit
AAGTAACACTTTAGATGAAAACACAAAGTCATGTATCATGCTAATTGAAA1020.10571153188446351No Hit
CAAAAGGCACTGATCCCGTGCTACCTGCAGGGGGGCTGCAATAGGCTTTG1020.10571153188446351No Hit
ACTTTGACTTATTCAGTTAACAGAGAGGACTAAAGGTGACAGTGATGGGG1020.10571153188446351No Hit
CTCATCCTCTGGTGCTATCGACCAAAACCCAAGCACTTTCCAAGAAAACC1000.10363875674947404No Hit
CTCCTGCGGTTCCCACTCCCAACAGGATGGAGCTGCCAAGACCAATCCCT1000.10363875674947404No Hit
GTTTGTAACAACAGAATCTACAGAAACAAGGTAATCAAAATCACACTTCT990.10260236918197929No Hit
GCATGGGGACCCTGCCTCTGTGGGTGCTTTTTCTTTTGACAGTGGCAAGG980.10156598161448455No Hit
GTGTAGAAGCTGTTCGGTGCAGTACCCTCAGTGGTCTCTTCCCCATGTTC980.10156598161448455No Hit
CACAGAGGCAGGGTCCCCATGCTGTGTCCCAAGACTGTCTCCTGATAAGA970.10052959404698981No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)