FastQCFastQC Report
Wed 12 May 2021
HGGF2BGXJ_n02_ReSeq_1971_d02_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGF2BGXJ_n02_ReSeq_1971_d02_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328152
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAATGGGCAGCATCTCGTATGCTCAGCAGCAGCAACAGCCTCAGTCT6820.20783051756503085No Hit
CTCGTATGCTCAGCAGCAGCAACAGCCTCAGTCTCAAGCTCCACAGCTTC6360.19381262341841585No Hit
GGGAATATGCAGGCTGCTGCTGCTGCGGCTGTGACTGAGGGTATGAAGAT5940.18101367658889783No Hit
GGAATATGCAGGCTGCTGCTGCTGCGGCTGTGACTGAGGGTATGAAGATG5210.1587678880518784No Hit
GCTTCAGACATCGCAACCTGCGTATTCTCAGCAGCAGTCTCAGCCACCCC4630.14109315195397254No Hit
GAATATGCAGGCTGCTGCTGCTGCGGCTGTGACTGAGGGTATGAAGATGA4000.1218947317096955No Hit
GTGTAAGACCCTACACTTGCTTTTGTTGAACCTCATCCGTTTTCTTACTG3970.12098052122187278No Hit
GTCTCAAGCTCCACAGCTTCAGACATCGCAACCTGCGTATTCTCAGCAGC3870.1179331529291304No Hit
ATGCAGGCTGCTGCTGCTGCGGCTGTGACTGAGGGTATGAAGATGACTGC3550.10818157439235476No Hit
GTGTAGAAGGTGGAGAAAGGAAACAAGCAATGCCTACAAAATGAGGACGA3460.10543894292888661No Hit
AGCGAAAGCAGGACGCAAAGTACAATGGGCAGCATCTCGTATGCTCAGCA3420.10421999561178966No Hit
GGATAAAGAAAAGGATACGCAAAAGAAGCGAACGGAAATTTCAGAGATAA3330.10147736414832151No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)