FastQCFastQC Report
Wed 12 May 2021
HGGF2BGXJ_n02_ReSeq_1970_d10_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGF2BGXJ_n02_ReSeq_1970_d10_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9037
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTGGAATGTAGGAGGTGATTTTTTGAAACGAACCAGAAATGCTTTTT580.6418059090406109No Hit
ATGTACTTGTGCTGGGAAGGACTTGGGCTGAAGAGGTGGTGGGAAGCCCC520.5754121943122718No Hit
GTGTGGGAGAGGGGCCATGACAGGGTGGGGGTCATTTCTGGGTGAAATTC480.5311497178267124No Hit
CTTCAGAACACCCCCAGCATCTCATCTTTAAGAGAAAACTCCACTTCTCC470.5200840987053226No Hit
CTATTAATTGTGACGAGGACTTCCACACCCTCCACGGTGCTGCGGCGCGT470.5200840987053226No Hit
GGTCAAGGCGGTTCAATGAGGAAGGACTTTTCTATTTGGGAAAGGAGAAA440.486887241341153No Hit
CCCCTAGCACTTGCAGAAGGCACCAGAAGGCCATCTCCGTGGGAGATACA430.4758216222197632No Hit
TGCTAGGGGTGATCAGAGCAGCCACTTCAGCGATGTACATCTTCAGAACA430.4758216222197632No Hit
GGCCATGACAGGGTGGGGGTCATTTCTGGGTGAAATTCAGGCCCAGCATT400.44262476485559366No Hit
CAAGTACATCATGCAACATCTTTAAAGAAGACTGCTCCTTTCTCCTTTCC390.4315591457342038No Hit
CCCTTCTACAGGCTAAAAGCAGCCTGGAAAAGGCATCAGCACCAGGTGTG380.42049352661281403No Hit
GCCTTGACCTGTATCTCCCACGGAGATGGCCTTCTGGTGCCTTCTGCAAG380.42049352661281403No Hit
GTGTTAATTTGTGCTCTATTAATTGTGACGAGGACTTCCACACCCTCCAC380.42049352661281403No Hit
ACCCAGGGAAGGATGTTAAGCAGTTCTGATTGAGAAGACATGAAAAGAAG360.3983622883700343No Hit
GGCTAAAAGCAGCCTGGAAAAGGCATCAGCACCAGGTGTGGACTTCAACA360.3983622883700343No Hit
CCCCCAGCATCTCATCTTTAAGAGAAAACTCCACTTCTCCCTTCTACAGG330.3651654310058648No Hit
CTTCCACACCCTCCACGGTGCTGCGGCGCGTGCACACATCAGTGTGGGAG330.3651654310058648No Hit
GTGCTGCGGCGCGTGCACACATCAGTGTGGGAGAGGGGCCATGACAGGGT320.3540998118844749No Hit
GGTTTGAGGAGTCACTGGGTGAGCTGGAAGTGAACAGGGGCATTTGTGCC310.3430341927630851No Hit
ATGTTAAGCAGTTCTGATTGAGAAGACATGAAAAGAAGTGCTGAGACTGT310.3430341927630851No Hit
GGTGTGGACTTCAACAAAAGCTCATTCAGAGGGGAATATGCTGGCAAAGG300.33196857364169524No Hit
GATAAGCTCAGTTGGTTGAGTGAAGGTTTGGGGGGCTACTGGACCCTTCT280.3098373353989156No Hit
GTGATGGAAGGATGGTTTGAGGAGTCACTGGGTGAGCTGGAAGTGAACAG280.3098373353989156No Hit
AAGCAGGACAGTGATGGAAGGATGGTTTGAGGAGTCACTGGGTGAGCTGG270.29877171627752575No Hit
TTCACAGCCTTTGCCAGCATATTCCCCTCTGAATGAGCTTTTGTTGAAGT270.29877171627752575No Hit
GTTCTGAAGATGTACATCGCTGAAGTGGCTGCTCTGATCACCCCTAGCAC260.2877060971561359No Hit
GCATTTCTGAACAAACGTTGAAATGGGCATTTCTGGAATGTAGGAGGTGA260.2877060971561359No Hit
CTTTAAAGATGTTGCATGATGTACTTGTGCTGGGAAGGACTTGGGCTGAA260.2877060971561359No Hit
GTGCTGAGACTGTGGATACAGATAAGCTCAGTTGGTTGAGTGAAGGTTTG250.276640478034746No Hit
GTTCTGATTGAGAAGACATGAAAAGAAGTGCTGAGACTGTGGATACAGAT250.276640478034746No Hit
GGGTGGGGGTCATTTCTGGGTGAAATTCAGGCCCAGCATTTCTGAACAAA250.276640478034746No Hit
TCCCTTCTCTGGGGGCTTCCCACCACCTCTTCAGCCCAAGTCCTTCCCAG250.276640478034746No Hit
CTTTAAAGAAGACTGCTCCTTTCTCCTTTCCCAAATAGAAAAGTCCTTCC240.2655748589133562No Hit
GTCCACACCTGGTGCTGATGCCTTTTCCAGGCTGCTTTTAGCCTGTAGAA240.2655748589133562No Hit
GTCACTGGGTGAGCTGGAAGTGAACAGGGGCATTTGTGCCACCCAGGGAA240.2655748589133562No Hit
ATACAGGTCAAGGCGGTTCAATGAGGAAGGACTTTTCTATTTGGGAAAGG240.2655748589133562No Hit
GTATCTCCCACGGAGATGGCCTTCTGGTGCCTTCTGCAAGTGCTAGGGGT240.2655748589133562No Hit
CCTTTCTCCTTTCCCAAATAGAAAAGTCCTTCCTCATTGAACCGCCTTGA230.2545092397919663No Hit
TCTTTAAAGAAGACTGCTCCTTTCTCCTTTCCCAAATAGAAAAGTCCTTC230.2545092397919663No Hit
GGACAGTGATGGAAGGATGGTTTGAGGAGTCACTGGGTGAGCTGGAAGTG230.2545092397919663No Hit
GAATGTAGGAGGTGATTTTTTGAAACGAACCAGAAATGCTTTTTTCTCCC230.2545092397919663No Hit
GGTCATTTCTGGGTGAAATTCAGGCCCAGCATTTCTGAACAAACGTTGAA220.2434436206705765No Hit
TTTCTGAACAAACGTTGAAATGGGCATTTCTGGAATGTAGGAGGTGATTT220.2434436206705765No Hit
GTGCTAGGGGTGATCAGAGCAGCCACTTCAGCGATGTACATCTTCAGAAC210.23237800154918667No Hit
CTACTGGACCCTTCTGGGTGCTCGTTCTTGTCTCACATCTCCCTCTCCAC210.23237800154918667No Hit
GTAGAAGGGAGAAGTGGAGTTTTCTCTTAAAGATGAGATGCTGGGGGTGT210.23237800154918667No Hit
TCCCACCACCTCTTCAGCCCAAGTCCTTCCCAGCACAAGTACATCATGCA210.23237800154918667No Hit
ATGTACATCTTCAGAACACCCCCAGCATCTCATCTTTAAGAGAAAACTCC210.23237800154918667No Hit
AACCAGAAATGCTTTTTTCTCCCGGCAGTGGCTTGACTTTCACAGCCTTT200.22131238242779683No Hit
TCCACACCCTCCACGGTGCTGCGGCGCGTGCACACATCAGTGTGGGAGAG200.22131238242779683No Hit
CTCCACGGTGCTGCGGCGCGTGCACACATCAGTGTGGGAGAGGGGCCATG200.22131238242779683No Hit
ACCCAGAATATAGCAAAGGATTTAAAGAGACATTTCTCCAAAGATATACA200.22131238242779683No Hit
CTTCAGCGATGTACATCTTCAGAACACCCCCAGCATCTCATCTTTAAGAG190.21024676330640701No Hit
GCATATTCCCCTCTGAATGAGCTTTTGTTGAAGTCCACACCTGGTGCTGA190.21024676330640701No Hit
GGAGAAAGGAGCAGTCTTCTTTAAAGATGTTGCATGATGTACTTGTGCTG190.21024676330640701No Hit
CCACAAACCCTCACCAGGATCCACAGACCTTCCCTTCTCTGGGGGCTTCC190.21024676330640701No Hit
CTTCTGGGTGCTCGTTCTTGTCTCACATCTCCCTCTCCACCTCCACAAAC190.21024676330640701No Hit
GGGGTGATCAGAGCAGCCACTTCAGCGATGTACATCTTCAGAACACCCCC190.21024676330640701No Hit
GACCTGTATCTCCCACGGAGATGGCCTTCTGGTGCCTTCTGCAAGTGCTA190.21024676330640701No Hit
CATCAGTGTGGGAGAGGGGCCATGACAGGGTGGGGGTCATTTCTGGGTGA180.19918114418501714No Hit
AGCTGGAAGTGAACAGGGGCATTTGTGCCACCCAGGGAAGGATGTTAAGC180.19918114418501714No Hit
AATTCAGGCCCAGCATTTCTGAACAAACGTTGAAATGGGCATTTCTGGAA170.1881155250636273No Hit
CTTCTACAGGCTAAAAGCAGCCTGGAAAAGGCATCAGCACCAGGTGTGGA170.1881155250636273No Hit
TCCCAGCACAAGTACATCATGCAACATCTTTAAAGAAGACTGCTCCTTTC170.1881155250636273No Hit
ACGTTGAAATGGGCATTTCTGGAATGTAGGAGGTGATTTTTTGAAACGAA170.1881155250636273No Hit
TCTCTGGGGGCTTCCCACCACCTCTTCAGCCCAAGTCCTTCCCAGCACAA170.1881155250636273No Hit
GATCAGAGCAGCCACTTCAGCGATGTACATCTTCAGAACACCCCCAGCAT170.1881155250636273No Hit
CCTGGTGCTGATGCCTTTTCCAGGCTGCTTTTAGCCTGTAGAAGGGAGAA160.17704990594223746No Hit
TTTCTGGGTGAAATTCAGGCCCAGCATTTCTGAACAAACGTTGAAATGGG160.17704990594223746No Hit
AAAGAAGACTGCTCCTTTCTCCTTTCCCAAATAGAAAAGTCCTTCCTCAT160.17704990594223746No Hit
TCATTGAACCGCCTTGACCTGTATCTCCCACGGAGATGGCCTTCTGGTGC160.17704990594223746No Hit
GTACATCTTCAGAACACCCCCAGCATCTCATCTTTAAGAGAAAACTCCAC160.17704990594223746No Hit
GTAGGAGGTGATTTTTTGAAACGAACCAGAAATGCTTTTTTCTCCCGGCA160.17704990594223746No Hit
GAATATAGCAAAGGATTTAAAGAGACATTTCTCCAAAGATATACAAATGG160.17704990594223746No Hit
TCTTTAAAGATGTTGCATGATGTACTTGTGCTGGGAAGGACTTGGGCTGA160.17704990594223746No Hit
GCACCAGGTGTGGACTTCAACAAAAGCTCATTCAGAGGGGAATATGCTGG150.16598428682084762No Hit
CTTCAGCCCAAGTCCTTCCCAGCACAAGTACATCATGCAACATCTTTAAA150.16598428682084762No Hit
AGCGAAAGCAGGTGGCAGCAAGCAGGACAGTGATGGAAGGATGGTTTGAG150.16598428682084762No Hit
GTTCAATGAGGAAGGACTTTTCTATTTGGGAAAGGAGAAAGGAGCAGTCT150.16598428682084762No Hit
GAGTAAGTATGCTGATGACACTAGGCTGAGACTGATAGTGAAAAGAGAAG150.16598428682084762No Hit
CGTTGAAATGGGCATTTCTGGAATGTAGGAGGTGATTTTTTGAAACGAAC150.16598428682084762No Hit
CTCCACTTCTCCCTTCTACAGGCTAAAAGCAGCCTGGAAAAGGCATCAGC150.16598428682084762No Hit
CCGTGGGAGATACAGGTCAAGGCGGTTCAATGAGGAAGGACTTTTCTATT150.16598428682084762No Hit
ATGTAGGAGGTGATTTTTTGAAACGAACCAGAAATGCTTTTTTCTCCCGG150.16598428682084762No Hit
GCCTTTGCCAGCATATTCCCCTCTGAATGAGCTTTTGTTGAAGTCCACAC150.16598428682084762No Hit
GACTTGGGCTGAAGAGGTGGTGGGAAGCCCCCAGAGAAGGGAAGGTCTGT150.16598428682084762No Hit
GTCTTCTTTAAAGATGTTGCATGATGTACTTGTGCTGGGAAGGACTTGGG140.1549186676994578No Hit
GAGATGGCCTTCTGGTGCCTTCTGCAAGTGCTAGGGGTGATCAGAGCAGC140.1549186676994578No Hit
GGGAAAAGACAGGAAACAGGTGTTGTGTTAATTTGTGCTCTATTAATTGT140.1549186676994578No Hit
TGACAGGGTGGGGGTCATTTCTGGGTGAAATTCAGGCCCAGCATTTCTGA140.1549186676994578No Hit
GAAAAGTCCTTCCTCATTGAACCGCCTTGACCTGTATCTCCCACGGAGAT140.1549186676994578No Hit
GTGGTGGGAAGCCCCCAGAGAAGGGAAGGTCTGTGGATCCTGGTGAGGGT140.1549186676994578No Hit
GTTCACAGACATTCGGTTTGTTTCTCCCTTTTGGTGATTGTGAGTAATGC140.1549186676994578No Hit
GAAAAGAAGTGCTGAGACTGTGGATACAGATAAGCTCAGTTGGTTGAGTG140.1549186676994578No Hit
GAAGATGTACATCGCTGAAGTGGCTGCTCTGATCACCCCTAGCACTTGCA140.1549186676994578No Hit
ATGCTACACCGTGGCTGATCCTCAAAACCCTCATTTATCTTAAGTAAAAG140.1549186676994578No Hit
GGCCCAGCATTTCTGAACAAACGTTGAAATGGGCATTTCTGGAATGTAGG140.1549186676994578No Hit
TCGCTGAAGTGGCTGCTCTGATCACCCCTAGCACTTGCAGAAGGCACCAG130.14385304857806794No Hit
CCCTCCACGGTGCTGCGGCGCGTGCACACATCAGTGTGGGAGAGGGGCCA130.14385304857806794No Hit
GGGAAGGATGTTAAGCAGTTCTGATTGAGAAGACATGAAAAGAAGTGCTG130.14385304857806794No Hit
CCAGAAGGCCATCTCCGTGGGAGATACAGGTCAAGGCGGTTCAATGAGGA130.14385304857806794No Hit
GGAATGTAGGAGGTGATTTTTTGAAACGAACCAGAAATGCTTTTTTCTCC130.14385304857806794No Hit
CCACTGGGATGGATAAAATTACTCAATAGAAAAATAACAAATAGTGGCAA130.14385304857806794No Hit
GTGGATACAGATAAGCTCAGTTGGTTGAGTGAAGGTTTGGGGGGCTACTG130.14385304857806794No Hit
ATGTTGCATGATGTACTTGTGCTGGGAAGGACTTGGGCTGAAGAGGTGGT130.14385304857806794No Hit
ATTCAGGCCCAGCATTTCTGAACAAACGTTGAAATGGGCATTTCTGGAAT130.14385304857806794No Hit
GACTGTGGATACAGATAAGCTCAGTTGGTTGAGTGAAGGTTTGGGGGGCT130.14385304857806794No Hit
GCCCAAGTCCTTCCCAGCACAAGTACATCATGCAACATCTTTAAAGAAGA130.14385304857806794No Hit
GATCACCCCTAGCACTTGCAGAAGGCACCAGAAGGCCATCTCCGTGGGAG130.14385304857806794No Hit
ATAGAACACAGTTCAATTGGGCTTCACCAAAATTAAAAACTTTGGTGCTT120.1327874294566781No Hit
GCCTTCTGGTGCCTTCTGCAAGTGCTAGGGGTGATCAGAGCAGCCACTTC120.1327874294566781No Hit
ATACAGATAAGCTCAGTTGGTTGAGTGAAGGTTTGGGGGGCTACTGGACC120.1327874294566781No Hit
GCACAAGTACATCATGCAACATCTTTAAAGAAGACTGCTCCTTTCTCCTT120.1327874294566781No Hit
CTCCCACGGAGATGGCCTTCTGGTGCCTTCTGCAAGTGCTAGGGGTGATC120.1327874294566781No Hit
GAAGTGAACAGGGGCATTTGTGCCACCCAGGGAAGGATGTTAAGCAGTTC120.1327874294566781No Hit
GCCTTTTCCAGGCTGCTTTTAGCCTGTAGAAGGGAGAAGTGGAGTTTTCT120.1327874294566781No Hit
GGGCATTTCTGGAATGTAGGAGGTGATTTTTTGAAACGAACCAGAAATGC120.1327874294566781No Hit
ATAGAAAAGTCCTTCCTCATTGAACCGCCTTGACCTGTATCTCCCACGGA120.1327874294566781No Hit
GGGCTGAAGAGGTGGTGGGAAGCCCCCAGAGAAGGGAAGGTCTGTGGATC120.1327874294566781No Hit
CTCTGGGGGCTTCCCACCACCTCTTCAGCCCAAGTCCTTCCCAGCACAAG110.12172181033528826No Hit
TTCCAGAAATGCCCATTTCAACGTTTGTTCAGAAATGCTGGGCCTGAATT110.12172181033528826No Hit
GTGGGGGTCATTTCTGGGTGAAATTCAGGCCCAGCATTTCTGAACAAACG110.12172181033528826No Hit
GGAAAAGACAGGAAACAGGTGTTGTGTTAATTTGTGCTCTATTAATTGTG110.12172181033528826No Hit
CAGTTGGTTGAGTGAAGGTTTGGGGGGCTACTGGACCCTTCTGGGTGCTC110.12172181033528826No Hit
GGTGGGGGTCATTTCTGGGTGAAATTCAGGCCCAGCATTTCTGAACAAAC110.12172181033528826No Hit
ATTCCCCTCTGAATGAGCTTTTGTTGAAGTCCACACCTGGTGCTGATGCC110.12172181033528826No Hit
TGCTGAGACTGTGGATACAGATAAGCTCAGTTGGTTGAGTGAAGGTTTGG110.12172181033528826No Hit
GTGTATGGATATATCACATATTATTTATCCATTTATCAGTTCACAGACAT110.12172181033528826No Hit
CCTATAGACATTCCCAGTCAGCAACTTAATGAGGGAGGAGCATTTCTCTC110.12172181033528826No Hit
GATGTACATCGCTGAAGTGGCTGCTCTGATCACCCCTAGCACTTGCAGAA110.12172181033528826No Hit
GAACAGGGGCATTTGTGCCACCCAGGGAAGGATGTTAAGCAGTTCTGATT110.12172181033528826No Hit
TGTAGGAGGTGATTTTTTGAAACGAACCAGAAATGCTTTTTTCTCCCGGC110.12172181033528826No Hit
ATTCAGAGGGGAATATGCTGGCAAAGGCTGTGAAAGTCAAGCCACTGCCG110.12172181033528826No Hit
ACCCCCAGCATCTCATCTTTAAGAGAAAACTCCACTTCTCCCTTCTACAG110.12172181033528826No Hit
CATCTTCAGAACACCCCCAGCATCTCATCTTTAAGAGAAAACTCCACTTC110.12172181033528826No Hit
CATGAAAAGAAGTGCTGAGACTGTGGATACAGATAAGCTCAGTTGGTTGA110.12172181033528826No Hit
CACCAGGTGTGGACTTCAACAAAAGCTCATTCAGAGGGGAATATGCTGGC100.11065619121389841No Hit
TCTCCCTTCTACAGGCTAAAAGCAGCCTGGAAAAGGCATCAGCACCAGGT100.11065619121389841No Hit
GGAATATGCTGGCAAAGGCTGTGAAAGTCAAGCCACTGCCGGGAGAAAAA100.11065619121389841No Hit
TTTCTGGTTCGTTTCAAAAAATCACCTCCTACATTCCAGAAATGCCCATT100.11065619121389841No Hit
GCTGCGGCGCGTGCACACATCAGTGTGGGAGAGGGGCCATGACAGGGTGG100.11065619121389841No Hit
CCCAGGGAAGGATGTTAAGCAGTTCTGATTGAGAAGACATGAAAAGAAGT100.11065619121389841No Hit
GTGTGGACTTCAACAAAAGCTCATTCAGAGGGGAATATGCTGGCAAAGGC100.11065619121389841No Hit
CATCAGCACCAGGTGTGGACTTCAACAAAAGCTCATTCAGAGGGGAATAT100.11065619121389841No Hit
GCCTGGAAAAGGCATCAGCACCAGGTGTGGACTTCAACAAAAGCTCATTC100.11065619121389841No Hit
GTCCAGTAGCCCCCCAAACCTTCACTCAACCAACTGAGCTTATCTGTATC100.11065619121389841No Hit
GGGGAATATGCTGGCAAAGGCTGTGAAAGTCAAGCCACTGCCGGGAGAAA100.11065619121389841No Hit
GCTTTTAGCCTGTAGAAGGGAGAAGTGGAGTTTTCTCTTAAAGATGAGAT100.11065619121389841No Hit
CTGCAAGTGCTAGGGGTGATCAGAGCAGCCACTTCAGCGATGTACATCTT100.11065619121389841No Hit
CTTTTAGATAACCTGATTTGAAAACATTTTCTCCTAGTGTGTTTGTCTTT100.11065619121389841No Hit
GTTCTATGTATGTGGAATGTCAGAATAGGCAAATCTAGAGAGGCACTAAG100.11065619121389841No Hit
GGATACAGATAAGCTCAGTTGGTTGAGTGAAGGTTTGGGGGGCTACTGGA100.11065619121389841No Hit
ATGACAGGGTGGGGGTCATTTCTGGGTGAAATTCAGGCCCAGCATTTCTG100.11065619121389841No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)