FastQCFastQC Report
Wed 12 May 2021
HGGF2BGXJ_n02_ReSeq_1970_d04_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGF2BGXJ_n02_ReSeq_1970_d04_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145029
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAAGGTCCTTTAGTTCAAGGTGCACATCCCGAATGCCTGTGGATCCG17281.1914858407628819No Hit
AGCGAAAGCAGGGAACCCGCGGCGATGGAGCGGCGGCGGGACTGGCGCTG11650.8032876183384012No Hit
GGGTAGGAAAGCGTATAAAGTGCAGATTGCCTTTACGTCCACACAGGGTA11460.7901867902281612No Hit
AAAGCAGGGAACCCGCGGCGATGGAGCGGCGGCGGGACTGGCGCTGGAAG10360.7143398906425611No Hit
GTCATGGGTAGGAAAGCGTATAAAGTGCAGATTGCCTTTACGTCCACACA9980.6881382344220811No Hit
TCCCGAATGCCTGTGGATCCGTAGGCCACGTTTGATGTCAGGTACTTGCG9980.6881382344220811No Hit
GACTGGAGCCGTGCGAGGGGGGCGGCGCAGCCTGCGGCCTTCGGCGAGAC9130.629529266560481No Hit
CGTCCACACAGGGTAAGGTCCTTTAGTTCAAGGTGCACATCCCGAATGCC7870.5426500906715209No Hit
CAGGGAACCCGCGGCGATGGAGCGGCGGCGGGACTGGCGCTGGAAGCGCG7520.5185169862579209No Hit
GCAGGGAACCCGCGGCGATGGAGCGGCGGCGGGACTGGCGCTGGAAGCGC6870.4736983637755207No Hit
GGATATCGGTGGGACCTTGGTCAAACTTGTCTATTTTGAACCAAAGGACA6450.44473863847920075No Hit
GCCTGCGGCCTTCGGCGAGACTCGGTCCGCAAGAACCGCCCGCTCTTCCC6320.4357749139827207No Hit
CATTACAGCTTCTCAAAAGCTCTTTAAAAACCACCGGAAGAACCCAATGT6300.4343958794448007No Hit
GCGTATAAAGTGCAGATTGCCTTTACGTCCACACAGGGTAAGGTCCTTTA6080.41922649952768065No Hit
GCGATGGAGCGGCGGCGGGACTGGCGCTGGAAGCGCGGCGGCGGGACTGG5680.3916458087692806No Hit
GGCATGTCATGGGTAGGAAAGCGTATAAAGTGCAGATTGCCTTTACGTCC5610.3868191878865606No Hit
GTGCGAGGGGGGCGGCGCAGCCTGCGGCCTTCGGCGAGACTCGGTCCGCA5460.3764764288521606No Hit
TCCCTTGGTTTGGCCTGGATATCGGTGGGACCTTGGTCAAACTTGTCTAT5250.3619965662040006No Hit
GGACTGGAGCCGTGCGAGGGGGGCGGCGCAGCCTGCGGCCTTCGGCGAGA5080.3502747726316806No Hit
GTAGGAAAGCGTATAAAGTGCAGATTGCCTTTACGTCCACACAGGGTAAG5050.3482062208248005No Hit
TCCGTAGGCCACGTTTGATGTCAGGTACTTGCGGATGTTTTTGAGGTTTT4760.32821022002496053No Hit
ATCTGGAACTTTCCCCTCCTTCTTTCTTCAGACCTCCTGGCCTGGGGAGG4620.3185569782595205No Hit
CGTTTGATGTCAGGTACTTGCGGATGTTTTTGAGGTTTTCTACTTCTTCG4220.2909762875011205No Hit
GTGGTATAACATGAATTTTCAAAAAGCAGGGGATTCCTGTAATTCCAAAT4210.29028677023216043No Hit
GCCTTTACGTCCACACAGGGTAAGGTCCTTTAGTTCAAGGTGCACATCCC4140.2854601493494405No Hit
AAAGCAGGCATGTCATGGGTAGGAAAGCGTATAAAGTGCAGATTGCCTTT4040.2785649766598404No Hit
AGCAGGGAACCCGCGGCGATGGAGCGGCGGCGGGACTGGCGCTGGAAGCG3950.2723593212392004No Hit
GAGCGGCGGCGGGACTGGCGCTGGAAGCGCGGCGGCGGGACTGGAGCCGT3770.2599480103979204No Hit
CGTATAAAGTGCAGATTGCCTTTACGTCCACACAGGGTAAGGTCCTTTAG3600.2482262168256004No Hit
GTGCACATCCCGAATGCCTGTGGATCCGTAGGCCACGTTTGATGTCAGGT3560.24546814774976036No Hit
CAGTAGGACAGGCCAGCGGCAGGAGAGGATTCAGGCCCCGTGAGGGAAGG3470.2392624923291204No Hit
TGTCATGGGTAGGAAAGCGTATAAAGTGCAGATTGCCTTTACGTCCACAC3470.2392624923291204No Hit
GGACTGGCGCTGGAAGCGCGGCGGCGGGACTGGAGCCGTGCGAGGGGGGC3450.23788345779120038No Hit
GTGTAGGTCTTTTGTGAAAGCAAAGTGGTATAACATGAATTTTCAAAAAG3350.23098828510160038No Hit
GCCCCAGGCTGCGCACAGAAAGGCAGGGGTTCTGGGGCACCTGGGTGGCT3310.22823021602576035No Hit
TCTCAGGACCCTGAGATCATAACCTGAGCCGAAGGCAGAGGCTCTAACCC3290.22685118148784034No Hit
ACCCGCGGCGATGGAGCGGCGGCGGGACTGGCGCTGGAAGCGCGGCGGCG3180.21926649152928032No Hit
GTCAGGTACTTGCGGATGTTTTTGAGGTTTTCTACTTCTTCGTCCTCCTC3040.20961324976384033No Hit
GGCGCTGGAAGCGCGGCGGCGGGACTGGAGCCGTGCGAGGGGGGCGGCGC2940.20271807707424033No Hit
TTCTACTTCTTCGTCCTCCTCTTCGGCAGTGATGTCCTTTGGTTCAAAAT2900.19996000799840033No Hit
GTCCGCAAGAACCGCCCGCTCTTCCCTTGGTTTGGCCTGGATATCGGTGG2870.19789145619152032No Hit
AGCAAAAGCAGGGAACCCGCGGCGATGGAGCGGCGGCGGGACTGGCGCTG2840.1958229043846403No Hit
GGTCAAACTTGTCTATTTTGAACCAAAGGACATCACTGCCGAAGAGGAGG2840.1958229043846403No Hit
GGGAACCCGCGGCGATGGAGCGGCGGCGGGACTGGCGCTGGAAGCGCGGC2790.19237531803984032No Hit
GTCCTGAGATGGAGCCCCGCATTGGGCTCTCTGCTCAGCGGGGAGCCTAC2750.1896172489640003No Hit
GGTTATGATCTCAGGGTCCTGAGATGGAGCCCCGCATTGGGCTCTCTGCT2740.1889277316950403No Hit
GCAAAGAGGTAGGCAGAGAGAGAGAGAGAAAGGGAAGTAGGCTCCCCGCT2740.1889277316950403No Hit
CTCTTCCCTTGGTTTGGCCTGGATATCGGTGGGACCTTGGTCAAACTTGT2700.1861696626192003No Hit
GTGGTAGAGATGATAAAAGGAAACATGAATAAATGTTTCCGATAAAAAAA2700.1861696626192003No Hit
AGCGAAAGCAGGCATGTCATGGGTAGGAAAGCGTATAAAGTGCAGATTGC2580.17789545539168028No Hit
ATTTTGAACCAAAGGACATCACTGCCGAAGAGGAGGACGAAGAAGTAGAA2580.17789545539168028No Hit
AGCGTATAAAGTGCAGATTGCCTTTACGTCCACACAGGGTAAGGTCCTTT2560.1765164208537603No Hit
AGTTCAAGGTGCACATCCCGAATGCCTGTGGATCCGTAGGCCACGTTTGA2540.1751373863158403No Hit
GCGAAAGCAGGGAACCCGCGGCGATGGAGCGGCGGCGGGACTGGCGCTGG2510.17306883450896027No Hit
ACTTTATATCCTTTAGATTTGCCAACTAGCATATGTGTCCATTACATACT2500.17237931724000027No Hit
GTATAACATGAATTTTCAAAAAGCAGGGGATTCCTGTAATTCCAAATAGC2440.16824221362624026No Hit
GTGATGTCCTTTGGTTCAAAATAGACAAGTTTGACCAAGGTCCCACCGAT2420.16686317908832027No Hit
GTGCAGATTGCCTTTACGTCCACACAGGGTAAGGTCCTTTAGTTCAAGGT2410.16617366181936027No Hit
GTCCTTTAGTTCAAGGTGCACATCCCGAATGCCTGTGGATCCGTAGGCCA2370.16341559274352027No Hit
GGCCTGGATATCGGTGGGACCTTGGTCAAACTTGTCTATTTTGAACCAAA2360.16272607547456025No Hit
GAAGAACCCAATGTAATCAGCAAAGATGGAGCAGCAGGAGGGGGAAGAGG2340.16134704093664026No Hit
GTGTATGTCCAAAGAAGATCCAGTTCATCATAGGCATTTGCTCCCTTCAG2340.16134704093664026No Hit
ATGGAGCGGCGGCGGGACTGGCGCTGGAAGCGCGGCGGCGGGACTGGAGC2330.16065752366768027No Hit
CCCTTGGTTTGGCCTGGATATCGGTGGGACCTTGGTCAAACTTGTCTATT2330.16065752366768027No Hit
CCTTTAGTTCAAGGTGCACATCCCGAATGCCTGTGGATCCGTAGGCCACG2330.16065752366768027No Hit
TCCCAGGCAAGCAACCTGGAGACAAAGCGATGAGCAGCCTCATCGTCTGG2300.15858897186080026No Hit
TTTTTGAGGTTTTCTACTTCTTCGTCCTCCTCTTCGGCAGTGATGTCCTT2300.15858897186080026No Hit
CGCTGGAAGCGCGGCGGCGGGACTGGAGCCGTGCGAGGGGGGCGGCGCAG2300.15858897186080026No Hit
GCTGTAATGTGATCAGCTTCTGGGCTTATTCCAGACGATGAGGCTGCTCA2270.15652042005392025No Hit
GGTTTGGCCTGGATATCGGTGGGACCTTGGTCAAACTTGTCTATTTTGAA2270.15652042005392025No Hit
GCCTTGACCTATCTGGAACTTTCCCCTCCTTCTTTCTTCAGACCTCCTGG2270.15652042005392025No Hit
AGTGTAGGTCTTTTGTGAAAGCAAAGTGGTATAACATGAATTTTCAAAAA2190.15100428190224024No Hit
GGATATACCATTTGGCCTAGTAATTTTACTTCTGCAAACCGATTCAATAG2170.14962524736432023No Hit
GTTCTGGGGCACCTGGGTGGCTCAGAGGGTTAGAGCCTCTGCCTTCGGCT2160.14893573009536024No Hit
CCTGTGGATCCGTAGGCCACGTTTGATGTCAGGTACTTGCGGATGTTTTT2150.14824621282640024No Hit
CTCTATATGTGAATAGAATCTCTTTACAGGAACAGACAGAAAGGCAACAG2120.14617766101952023No Hit
CTTGAATACATGGTAAACTTTTTTGCTCTCATAGAGCCTGGACTAGATTT2110.14548814375056024No Hit
CTCATAGAGCCTGGACTAGATTTTATTTGCTTTTGAGTGCCTTTTTGAAA2110.14548814375056024No Hit
GTCTGGAATAAGCCCAGAAGCTGATCACATTACAGCTTCTCAAAAGCTCT2100.14479862648160022No Hit
GACTGGCGCTGGAAGCGCGGCGGCGGGACTGGAGCCGTGCGAGGGGGGCG2080.14341959194368023No Hit
CTGTGGATCCGTAGGCCACGTTTGATGTCAGGTACTTGCGGATGTTTTTG2040.14066152286784023No Hit
CTCAAGGCCTTGACCTATCTGGAACTTTCCCCTCCTTCTTTCTTCAGACC2040.14066152286784023No Hit
GACATACACAGCTCAAAAAAGAAAGTCCCACTGAAGCAGCTTTCAAAAAG2040.14066152286784023No Hit
GAGCAAAAGCAGGGAACCCGCGGCGATGGAGCGGCGGCGGGACTGGCGCT2030.13997200559888023No Hit
GAGGTAGGCAGAGAGAGAGAGAGAAAGGGAAGTAGGCTCCCCGCTGAGCA2030.13997200559888023No Hit
CATCCCGAATGCCTGTGGATCCGTAGGCCACGTTTGATGTCAGGTACTTG2010.13859297106096022No Hit
TCTTCGTCCTCCTCTTCGGCAGTGATGTCCTTTGGTTCAAAATAGACAAG2000.13790345379200022No Hit
GAACCCGCGGCGATGGAGCGGCGGCGGGACTGGCGCTGGAAGCGCGGCGG1990.13721393652304023No Hit
TGTCTATTTTGAACCAAAGGACATCACTGCCGAAGAGGAGGACGAAGAAG1980.1365244192540802No Hit
GTGGGACCTTGGTCAAACTTGTCTATTTTGAACCAAAGGACATCACTGCC1970.13583490198512022No Hit
CTCTTCGGCAGTGATGTCCTTTGGTTCAAAATAGACAAGTTTGACCAAGG1950.13445586744720023No Hit
ACCCAATGTAATCAGCAAAGATGGAGCAGCAGGAGGGGGAAGAGGAAGAG1940.1337663501782402No Hit
GCGGCGGGACTGGCGCTGGAAGCGCGGCGGCGGGACTGGAGCCGTGCGAG1920.13238731564032022No Hit
CTTCGGCAGTGATGTCCTTTGGTTCAAAATAGACAAGTTTGACCAAGGTC1920.13238731564032022No Hit
CGGTGGGACCTTGGTCAAACTTGTCTATTTTGAACCAAAGGACATCACTG1900.1310082811024002No Hit
GGGCGGCGCAGCCTGCGGCCTTCGGCGAGACTCGGTCCGCAAGAACCGCC1870.1289397292955202No Hit
GCCGAAGAGGAGGACGAAGAAGTAGAAAACCTCAAAAACATCCGCAAGTA1860.1282502120265602No Hit
GGCCTGGGGAGGATGAAGATGCATGTCCCAGGCAAGCAACCTGGAGACAA1860.1282502120265602No Hit
GGACATCACTGCCGAAGAGGAGGACGAAGAAGTAGAAAACCTCAAAAACA1820.1254921429507202No Hit
ATATAGTACACATGTATTTAGTGAACTGTCCTCTTTACATGATGGTAGCG1810.1248026256817602No Hit
ACATTGGGTTCTTCCGGTGGTTTTTAAAGAGCTTTTGAGAAGCTGTAATG1810.1248026256817602No Hit
CCTCTTCGGCAGTGATGTCCTTTGGTTCAAAATAGACAAGTTTGACCAAG1790.12342359114384019No Hit
GGCCAGCGGCAGGAGAGGATTCAGGCCCCGTGAGGGAAGGAAATCACCAG1780.12273407387488018No Hit
CTCCTGGCCTGGGGAGGATGAAGATGCATGTCCCAGGCAAGCAACCTGGA1770.1220445566059202No Hit
CATATATAGTACACATGTATTTAGTGAACTGTCCTCTTTACATGATGGTA1740.11997600479904018No Hit
AAGGTGCACATCCCGAATGCCTGTGGATCCGTAGGCCACGTTTGATGTCA1730.1192864875300802No Hit
CATAGAGCCTGGACTAGATTTTATTTGCTTTTGAGTGCCTTTTTGAAAGC1710.11790745299216018No Hit
GGATTCCTGTAATTCCAAATAGCCAGTAGGACAGGCCAGCGGCAGGAGAG1700.1172179357232002No Hit
GCAAAAGCAGGGAACCCGCGGCGATGGAGCGGCGGCGGGACTGGCGCTGG1700.1172179357232002No Hit
ATAAAGTGCAGATTGCCTTTACGTCCACACAGGGTAAGGTCCTTTAGTTC1690.1165284184542402No Hit
GTCCATTACATACTCAAGGCCTTGACCTATCTGGAACTTTCCCCTCCTTC1690.1165284184542402No Hit
GGATGGGACTGAGAAACAGATCTACTTTATATCCTTTAGATTTGCCAACT1680.11583890118528019No Hit
GAAGAAAGGCAGGGCTTCTAAGGGCCCGGTCTCTGGAGCCTTCTGCTGCC1670.11514938391632018No Hit
GGGTTCTTCCGGTGGTTTTTAAAGAGCTTTTGAGAAGCTGTAATGTGATC1670.11514938391632018No Hit
GCTTCTAAGGGCCCGGTCTCTGGAGCCTTCTGCTGCCAGGGAAAAGAATT1660.11445986664736019No Hit
GTTCTAGGATACTCAGTTAAGTATTGTTGGGATGGCAAGATTGTATAGCC1630.11239131484048018No Hit
GCCTTCGGCGAGACTCGGTCCGCAAGAACCGCCCGCTCTTCCCTTGGTTT1630.11239131484048018No Hit
GAGCGAAAGCAGGGAACCCGCGGCGATGGAGCGGCGGCGGGACTGGCGCT1620.11170179757152018No Hit
GTTTTCTACTTCTTCGTCCTCCTCTTCGGCAGTGATGTCCTTTGGTTCAA1620.11170179757152018No Hit
CTCTGGAGCCTTCTGCTGCCAGGGAAAAGAATTCCTCTTCCTCTTCCCCC1620.11170179757152018No Hit
TGCGAGGGGGGCGGCGCAGCCTGCGGCCTTCGGCGAGACTCGGTCCGCAA1620.11170179757152018No Hit
AAGCAGGGAACCCGCGGCGATGGAGCGGCGGCGGGACTGGCGCTGGAAGC1610.11101228030256018No Hit
GTCAAAGACTGACCTTATTAAGAAAGCAGTGACCTCTTGAATACATGGTA1610.11101228030256018No Hit
TCACATTACAGCTTCTCAAAAGCTCTTTAAAAACCACCGGAAGAACCCAA1600.11032276303360017No Hit
GGCTATAGGAAATATTTTGAGCAGCAGAGGTGGGTTGGATTTGGAAAGGG1600.11032276303360017No Hit
CGCAAGAACCGCCCGCTCTTCCCTTGGTTTGGCCTGGATATCGGTGGGAC1600.11032276303360017No Hit
TTTGATGTCAGGTACTTGCGGATGTTTTTGAGGTTTTCTACTTCTTCGTC1590.10963324576464016No Hit
GCTTTGGTGGGTGGCAGCTGGGAGCCTTTATGGCCCCAGGCTGCGCACAG1580.10894372849568018No Hit
ACCTTGGTCAAACTTGTCTATTTTGAACCAAAGGACATCACTGCCGAAGA1580.10894372849568018No Hit
CGGCGATGGAGCGGCGGCGGGACTGGCGCTGGAAGCGCGGCGGCGGGACT1560.10756469395776017No Hit
CTTTAAAAACCACCGGAAGAACCCAATGTAATCAGCAAAGATGGAGCAGC1540.10618565941984018No Hit
GTACTTGCGGATGTTTTTGAGGTTTTCTACTTCTTCGTCCTCCTCTTCGG1540.10618565941984018No Hit
GGATGAAGATGCATGTCCCAGGCAAGCAACCTGGAGACAAAGCGATGAGC1540.10618565941984018No Hit
GTACTATACATTTCATTCCCTTCCATACTGGTTTTCATTGATCTTAGGAA1540.10618565941984018No Hit
GTCCAGCGCGCGGTCAATGTCGGGGATGCGGTCAAGGCGGGACAGCTGAT1530.10549614215088017No Hit
GGTATAACATGAATTTTCAAAAAGCAGGGGATTCCTGTAATTCCAAATAG1530.10549614215088017No Hit
CCGATATCCAGGCCAAACCAAGGGAAGAGCGGGCGGTTCTTGCGGACCGA1520.10480662488192016No Hit
CTTTAGTTCAAGGTGCACATCCCGAATGCCTGTGGATCCGTAGGCCACGT1510.10411710761296016No Hit
TACTAAAAGGATATACCATTTGGCCTAGTAATTTTACTTCTGCAAACCGA1510.10411710761296016No Hit
GTACGAGTGTAGGTCTTTTGTGAAAGCAAAGTGGTATAACATGAATTTTC1490.10273807307504017No Hit
CCTTTAGATTTGCCAACTAGCATATGTGTCCATTACATACTCAAGGCCTT1480.10204855580608016No Hit
GAATAAGCCCAGAAGCTGATCACATTACAGCTTCTCAAAAGCTCTTTAAA1480.10204855580608016No Hit
GCCCAGAAGCTGATCACATTACAGCTTCTCAAAAGCTCTTTAAAAACCAC1460.10066952126816017No Hit
CCTGGAGACAAAGCGATGAGCAGCCTCATCGTCTGGAATAAGCCCAGAAG1460.10066952126816017No Hit
GAATAAATGTTTCCGATAAAAAAAGCTGATATACCTGTTTGTATTTCTGT1460.10066952126816017No Hit
GGCCACGTTTGATGTCAGGTACTTGCGGATGTTTTTGAGGTTTTCTACTT1460.10066952126816017No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)