FastQCFastQC Report
Wed 12 May 2021
HGGF2BGXJ_n02_ReSeq_1912_d04_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGF2BGXJ_n02_ReSeq_1912_d04_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1543354
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC44800.2902768904606461No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGATTCCAACACTGTGTCAAG22940.14863731846355407No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT21840.14150998409956497No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA17940.1162403440817855No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT17860.11572199249167722No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT16030.10386469986794994No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTTTACTTT15910.10308717248278748No Hit
TTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCTTCAAAC15610.10114335401988138No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)