FastQCFastQC Report
Wed 12 May 2021
HGGF2BGXJ_n02_ReSeq_1419_d02_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGF2BGXJ_n02_ReSeq_1419_d02_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5016
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATAGAGTGATGGTTACACGCATAATAAATCCTAAAACATGTGTCCGG100.1993620414673046No Hit
CTTTAGAAGTTACTGAAAAACTGTTAACTTTTGAATATACTCACTCTTCA100.1993620414673046No Hit
GTATGTAACATAGCCTTATGTTGAATTTCTTCAGCATCTACACCACCTTG80.1594896331738437No Hit
ATCATATGGTAATCCTCTTTTAAATTTTTTGAGAATCCTCATATTCTTTC70.13955342902711323No Hit
CTTCTAGCACATTATTAATGAGGATTCAGATAATGCTTTCAAAAGGAAAG60.11961722488038277No Hit
TTCTCATTCTGATAAGAATTACCTTGGTTCCTGGAGAAGAGCAGGTAGAA60.11961722488038277No Hit
CCAGAAATCTGTCCTAAGAAACTAACACCTAGAAATAAAAGTGATGCAAT60.11961722488038277No Hit
TCTCAGGTGTTCCTTATGATTCCAAGTTAAGCCATAAACAATAATGAGCC60.11961722488038277No Hit
AATATAGTCACATTTAGCATGAATCAAGGTTTTGGGGATAATACGAATTT60.11961722488038277No Hit
GATTGTTTTAGAATGTTAACTCATCTATATGGGCACTCAGAAATTTCAGT60.11961722488038277No Hit
CCCAAAATGAACATATACTTTGAGCAACTCAAACTTCAACTGAAATTATT60.11961722488038277No Hit
CATTTAGCATGAATCAAGGTTTTGGGGATAATACGAATTTTTAGAAGGCC60.11961722488038277No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)