FastQCFastQC Report
Wed 12 May 2021
HGGF2BGXJ_n02_ReSeq_1411_d12_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGF2BGXJ_n02_ReSeq_1411_d12_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1932624
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGACGGATAAGCAAGAGTAGGCTGGAGCAAACGCGCCAG36890.19088037818013232No Hit
GAGCAAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATC31480.16288734901356913No Hit
GGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGAT25830.13365248491170553No Hit
GGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACG25700.13297982432175115No Hit
GTCTTCCGACTGGGCCTTTCGCTTAATTTGATGCCTGGCAGTTCCCTACT20150.10426239144292941No Hit
GTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCTTCGGCGTTGT20000.10348624460836667No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)